Larvae of Drosophila melanogaster reared at 23°C and switched to 14°C for 1 h are 0.5°C warmer than the surrounding medium. In keeping with dissipation of energy, respiration of Drosophila melanogaster larvae cannot be decreased by the F-ATPase inhibitor oligomycin or stimulated by protonophore. Silencing of Ucp4C conferred sensitivity of respiration to oligomycin and uncoupler, and prevented larva-to-adult progression at 15°C but not 23°C. Uncoupled respiration of larval mitochondria required palmitate, was dependent on Ucp4C and was inhibited by guanosine diphosphate. UCP4C is required for development through the prepupal stages at low temperatures and may be an uncoupling protein. Synopsis Larvae of Drosophila melanogaster kept in the cold are warmer than the surrounding medium. In keeping with dissipation of energy, respiration of larvae is uncoupled. Knockdown of UCP4C, a homolog of the mammalian uncoupling protein UCP1, confers larvae with coupled respiration. UCP4C is required for development through the prepupal stages at low temperature. Larvae of Drosophila melanogaster kept in the cold are warmer than the surrounding medium. In keeping with dissipation of energy, respiration of larvae is uncoupled. © 2014 The Authors.
Da-Re C., De Pitta C., Zordan M.A., Teza G., Nestola F., Zeviani M., et al. (2014). UCP4C mediates uncoupled respiration in larvae of Drosophila melanogaster. EMBO REPORTS, 15(5), 586-591 [10.1002/embr.201337972].
UCP4C mediates uncoupled respiration in larvae of Drosophila melanogaster
Teza G.;
2014
Abstract
Larvae of Drosophila melanogaster reared at 23°C and switched to 14°C for 1 h are 0.5°C warmer than the surrounding medium. In keeping with dissipation of energy, respiration of Drosophila melanogaster larvae cannot be decreased by the F-ATPase inhibitor oligomycin or stimulated by protonophore. Silencing of Ucp4C conferred sensitivity of respiration to oligomycin and uncoupler, and prevented larva-to-adult progression at 15°C but not 23°C. Uncoupled respiration of larval mitochondria required palmitate, was dependent on Ucp4C and was inhibited by guanosine diphosphate. UCP4C is required for development through the prepupal stages at low temperatures and may be an uncoupling protein. Synopsis Larvae of Drosophila melanogaster kept in the cold are warmer than the surrounding medium. In keeping with dissipation of energy, respiration of larvae is uncoupled. Knockdown of UCP4C, a homolog of the mammalian uncoupling protein UCP1, confers larvae with coupled respiration. UCP4C is required for development through the prepupal stages at low temperature. Larvae of Drosophila melanogaster kept in the cold are warmer than the surrounding medium. In keeping with dissipation of energy, respiration of larvae is uncoupled. © 2014 The Authors.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
