We determined HSP70 and HSP90 expression by Western blot and their cellular distribution by immunofluorescence in ejaculated, capacitated and acrosome-reacted boar spermatozoa. The expression of both HSPs was not different among the three types of sperms. HSP90 fluorescence was localized in the midpiece of the three groups of sperms, while permeabilized ejaculated sperms showed the HSP70 signal in a cone-shaped region in the equatorial segment. This localization underwent several rearrangements following capacitation and acrosome reaction (AR). In non-permeabilized cells the HSP70 signal was present only in few ejaculated sperms, while an increased percentage of positive cells was recorded after capacitation and AR. Our results indicate that during capacitation and AR a relocalization of HSP70 occurs. This protein is probably translocated from the inner to the outer leaflet of the membrane suggesting a possible role in sperm-oocyte interaction.
Volpe S., Spinaci M., De Ambrogi M., Bernardini C., Tamanini C., Galeati G. (2004). Localizzazione della HSP70 e della HSP90 negli spermatozoi di verro. s.l : s.n.
Localizzazione della HSP70 e della HSP90 negli spermatozoi di verro
VOLPE, SARA;SPINACI, MARCELLA;DE AMBROGI, MARCO;BERNARDINI, CHIARA;TAMANINI, CARLO;GALEATI, GIOVANNA
2004
Abstract
We determined HSP70 and HSP90 expression by Western blot and their cellular distribution by immunofluorescence in ejaculated, capacitated and acrosome-reacted boar spermatozoa. The expression of both HSPs was not different among the three types of sperms. HSP90 fluorescence was localized in the midpiece of the three groups of sperms, while permeabilized ejaculated sperms showed the HSP70 signal in a cone-shaped region in the equatorial segment. This localization underwent several rearrangements following capacitation and acrosome reaction (AR). In non-permeabilized cells the HSP70 signal was present only in few ejaculated sperms, while an increased percentage of positive cells was recorded after capacitation and AR. Our results indicate that during capacitation and AR a relocalization of HSP70 occurs. This protein is probably translocated from the inner to the outer leaflet of the membrane suggesting a possible role in sperm-oocyte interaction.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
