Eight strains of an unknown thermotolerant Campylobacter species were isolated from the caecal contents of rabbits (Oryctolagus cuniculus). All strains were initially identified as belonging to the genus Campylobacter by means of genus-specific PCR, but none were identified using species-specific PCR for known thermophilic species. Cells were spiral shaped with bipolar unsheathed flagella, with no periplasmic fibres, and appeared coccoid after 10–12 days of incubation. Phylogenetic analyses based on 16S rRNA gene, rpoB and groEL sequences revealed that all strains formed a robust clade that was very distinct from recognized Campylobacter species. 16S rRNA gene sequence pairwise comparisons of strain 150BT with the type strains of other Campylobacter species revealed that the nearest phylogenetic neighbour was Campylobacter helveticus NCTC 12470T, with 96.6 % similarity. The uniqueness of these rabbit isolates was confirmed by whole-cell protein electrophoresis. Taken together, these data indicate that the strains belong to a novel Campylobacter species for which the name Campylobacter cuniculorum sp. nov. is proposed, with 150BT (=LMG 24588T =CCUG 56289T) as the type strain.
Campylobacter cuniculorum sp. nov., from rabbits / Zanoni R.G.; Debruyne L.; Rossi M.; Revez J.; Vandamme P.. - In: INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. - ISSN 1466-5026. - STAMPA. - 59:(2009), pp. 1666-1671. [10.1099/ijs.0.007286-0]
Campylobacter cuniculorum sp. nov., from rabbits.
ZANONI, RENATO GIULIO;ROSSI, MIRKO;REVEZ, JOANA MARREIROS CABRITA;
2009
Abstract
Eight strains of an unknown thermotolerant Campylobacter species were isolated from the caecal contents of rabbits (Oryctolagus cuniculus). All strains were initially identified as belonging to the genus Campylobacter by means of genus-specific PCR, but none were identified using species-specific PCR for known thermophilic species. Cells were spiral shaped with bipolar unsheathed flagella, with no periplasmic fibres, and appeared coccoid after 10–12 days of incubation. Phylogenetic analyses based on 16S rRNA gene, rpoB and groEL sequences revealed that all strains formed a robust clade that was very distinct from recognized Campylobacter species. 16S rRNA gene sequence pairwise comparisons of strain 150BT with the type strains of other Campylobacter species revealed that the nearest phylogenetic neighbour was Campylobacter helveticus NCTC 12470T, with 96.6 % similarity. The uniqueness of these rabbit isolates was confirmed by whole-cell protein electrophoresis. Taken together, these data indicate that the strains belong to a novel Campylobacter species for which the name Campylobacter cuniculorum sp. nov. is proposed, with 150BT (=LMG 24588T =CCUG 56289T) as the type strain.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
