Previous research suggests that human basophil activation may be inhibited by histamine even at extremely low doses (high dilutions). In our experiment, membrane up-regulation of CD203c, which proved to be a more consistent activation marker than CD63, was significantly inhibited in samples treated with histamine at the dilutions of 2C, 12C, 14C, 15C and 16C. Control water dilutions/succussions did not show any significant effect. Therefore, using a strictly standardized flow cytometry protocol and a new dilution/succussion procedure, we have shown that low and high dilution of histamine do inhibit CD203c up-regulation in anti-IgE stimulated basophils.

S. Chirumbolo, M. Brizzi, R. Ortolani, A. Vella, P. Bellavite (2009). Inhibition of CD203c membrane up-regulation in human basophils by high dilutions of histamine: a controlled replication study. INFLAMMATION RESEARCH, 58, 755-764.

Inhibition of CD203c membrane up-regulation in human basophils by high dilutions of histamine: a controlled replication study.

BRIZZI, MAURIZIO;
2009

Abstract

Previous research suggests that human basophil activation may be inhibited by histamine even at extremely low doses (high dilutions). In our experiment, membrane up-regulation of CD203c, which proved to be a more consistent activation marker than CD63, was significantly inhibited in samples treated with histamine at the dilutions of 2C, 12C, 14C, 15C and 16C. Control water dilutions/succussions did not show any significant effect. Therefore, using a strictly standardized flow cytometry protocol and a new dilution/succussion procedure, we have shown that low and high dilution of histamine do inhibit CD203c up-regulation in anti-IgE stimulated basophils.
2009
S. Chirumbolo, M. Brizzi, R. Ortolani, A. Vella, P. Bellavite (2009). Inhibition of CD203c membrane up-regulation in human basophils by high dilutions of histamine: a controlled replication study. INFLAMMATION RESEARCH, 58, 755-764.
S. Chirumbolo; M. Brizzi; R. Ortolani; A. Vella; P. Bellavite
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/85246
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