Field trials were carried out in 1998/99, 2003/04 and 2006/07 at the Experimental Farm of the Cereals Research Institute near Rome in a field with natural inoculum sources of both Wheat spindle streak mosaic virus (WSSMV) and Soil-borne cereal mosaic Virus (SBCMV) to evaluate the reaction to WSSMV of 71 cultivars of durum wheat (Triticum durum ) marketed in Italy. Reactions to WSSMV were evaluated on foliar extracts using DAS (Double Antibody Sandwich) ELISA. Nine of these 71 cvs. were grown over three seasons, 18 cvs. over two seasons, and 44 in one season only. In each of the trials, the DAS-ELISA values obtained for single cultivars were indexed in order to allow comparisons between different years. Cultivar Claudio, assayed over three seasons, showed the lowest overall mean ELISA index (0.5%), and thus appears – at present - the safest choice for soils with WSSMV in Italy. Relatively low ELISA indexes were observed also for cvs. Colorado, Rusticano and Provenzal, tested two seasons only, and for cvs. Tiziana, Italo, Ofanto and Dupri, assayed one season. Cultivars Colorado, Provenzal, Tiziana and Dupri are of particular interest, in that they are known to possess also a high degree of resistance towards SBCMV in Italy, whereas the highly WSSMV-resistant cv. Claudio is quite susceptible to SBWMV
Reaction of seventy-one cultivars of Triticum durum Desf. to Wheat spindle streak mosaic virus in central Italy
RATTI, CLAUDIO;RUBIES AUTONELL, CONCEPCION
2008
Abstract
Field trials were carried out in 1998/99, 2003/04 and 2006/07 at the Experimental Farm of the Cereals Research Institute near Rome in a field with natural inoculum sources of both Wheat spindle streak mosaic virus (WSSMV) and Soil-borne cereal mosaic Virus (SBCMV) to evaluate the reaction to WSSMV of 71 cultivars of durum wheat (Triticum durum ) marketed in Italy. Reactions to WSSMV were evaluated on foliar extracts using DAS (Double Antibody Sandwich) ELISA. Nine of these 71 cvs. were grown over three seasons, 18 cvs. over two seasons, and 44 in one season only. In each of the trials, the DAS-ELISA values obtained for single cultivars were indexed in order to allow comparisons between different years. Cultivar Claudio, assayed over three seasons, showed the lowest overall mean ELISA index (0.5%), and thus appears – at present - the safest choice for soils with WSSMV in Italy. Relatively low ELISA indexes were observed also for cvs. Colorado, Rusticano and Provenzal, tested two seasons only, and for cvs. Tiziana, Italo, Ofanto and Dupri, assayed one season. Cultivars Colorado, Provenzal, Tiziana and Dupri are of particular interest, in that they are known to possess also a high degree of resistance towards SBCMV in Italy, whereas the highly WSSMV-resistant cv. Claudio is quite susceptible to SBWMVI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
