A whole virus, inactivated, Porcine Circovirus 2b (PCV2b) vaccine was submitted to a quantal assay of potency, as explained in detail in our companion paper [1]. To this purpose, twenty, 45-day old piglets, checked for maternally-derived antibody (MDA), were allocated to four groups of 5 animals each; these were vaccinated with 800/266/88/0 nanograms, respectively, of an inactivated PCV2b strain, consisting of two distinct virion populations. Twenty-six days later, all the pigs were challenged intranasally with the homologous PCV2b strain. In the presence of a clear dose-dependent protection in terms of viremia, no such effect was observed in terms of weight gain after challenge. The 800 and 266-ng payloads were associated with neutralizing antibody titers above the MDA levels in oral fluids. Higher levels of viremia in control and 88-ng groups [1] coincided with a higher Natural Killer activity of tracheobronchial lymph node cells from PCV2-infected pigs. The PCV2 ORF2-specific ELISPOT assay for IFN-g– secreting cells showed very few (2–4) ORF2-specific cells/105 peripheral blood mononuclear cells beyond the basal levels under our experimental conditions (non-significant differences among groups). Also, no significant differences were observed in the degree of lymphoid tissue hyperplasia among the different groups.

Guarneri F., Tresoldi E.T., Sarli G., Boniotti M.B., Lelli D., Barbieri I., et al. (2021). Dataset of immune responses induced in swine by an inactivated Porcine Circovirus 2b vaccine. DATA IN BRIEF, 35, 106906-106906 [10.1016/j.dib.2021.106906].

Dataset of immune responses induced in swine by an inactivated Porcine Circovirus 2b vaccine

Sarli G.;Bacci B.;D'Annunzio G.;
2021

Abstract

A whole virus, inactivated, Porcine Circovirus 2b (PCV2b) vaccine was submitted to a quantal assay of potency, as explained in detail in our companion paper [1]. To this purpose, twenty, 45-day old piglets, checked for maternally-derived antibody (MDA), were allocated to four groups of 5 animals each; these were vaccinated with 800/266/88/0 nanograms, respectively, of an inactivated PCV2b strain, consisting of two distinct virion populations. Twenty-six days later, all the pigs were challenged intranasally with the homologous PCV2b strain. In the presence of a clear dose-dependent protection in terms of viremia, no such effect was observed in terms of weight gain after challenge. The 800 and 266-ng payloads were associated with neutralizing antibody titers above the MDA levels in oral fluids. Higher levels of viremia in control and 88-ng groups [1] coincided with a higher Natural Killer activity of tracheobronchial lymph node cells from PCV2-infected pigs. The PCV2 ORF2-specific ELISPOT assay for IFN-g– secreting cells showed very few (2–4) ORF2-specific cells/105 peripheral blood mononuclear cells beyond the basal levels under our experimental conditions (non-significant differences among groups). Also, no significant differences were observed in the degree of lymphoid tissue hyperplasia among the different groups.
2021
Guarneri F., Tresoldi E.T., Sarli G., Boniotti M.B., Lelli D., Barbieri I., et al. (2021). Dataset of immune responses induced in swine by an inactivated Porcine Circovirus 2b vaccine. DATA IN BRIEF, 35, 106906-106906 [10.1016/j.dib.2021.106906].
Guarneri F.; Tresoldi E.T.; Sarli G.; Boniotti M.B.; Lelli D.; Barbieri I.; Bacci B.; D'Annunzio G.; Amadori M.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/836637
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