Mycoplasma lipoproteins play a relevant role in pathogenicity and directly interact with the host immune system. Among human mycoplasmas, Mycoplasma hominis is described as a commensal bacterium that can be associated with a number of genital and extragenital conditions. Mechanisms of M. hominis pathogenicity are still largely obscure, and only a limited number of proteins have been associated with virulence. The current study focused on investigating the role of MHO-0730 as a virulence factor and demonstrated that MHO-0730 is a surface lipoprotein, potentially expressed in vivo during natural infection, acting both as a nuclease with its amino acidic portion and as a potent inducer of Neutrophil extracellular trapsosis with its N-terminal lipid moiety. Evidence for M. hominis neutrophil extracellular trap escape is also presented. Results highlight the relevance of MHO-0730 in promoting infection and modulation and evasion of innate immunity and provide additional knowledge on M. hominis virulence and survival in the host.

Cacciotto C., Dessi D., Cubeddu T., Cocco A.R., Pisano A., Tore G., et al. (2019). MHO-0730 as a Surface-Exposed Calcium-Dependent Nuclease of Mycoplasma hominis Promoting Neutrophil Extracellular Trap Formation and Escape. THE JOURNAL OF INFECTIOUS DISEASES, 220(12), 1999-2008 [10.1093/infdis/jiz406].

MHO-0730 as a Surface-Exposed Calcium-Dependent Nuclease of Mycoplasma hominis Promoting Neutrophil Extracellular Trap Formation and Escape

Cacciotto C.;
2019

Abstract

Mycoplasma lipoproteins play a relevant role in pathogenicity and directly interact with the host immune system. Among human mycoplasmas, Mycoplasma hominis is described as a commensal bacterium that can be associated with a number of genital and extragenital conditions. Mechanisms of M. hominis pathogenicity are still largely obscure, and only a limited number of proteins have been associated with virulence. The current study focused on investigating the role of MHO-0730 as a virulence factor and demonstrated that MHO-0730 is a surface lipoprotein, potentially expressed in vivo during natural infection, acting both as a nuclease with its amino acidic portion and as a potent inducer of Neutrophil extracellular trapsosis with its N-terminal lipid moiety. Evidence for M. hominis neutrophil extracellular trap escape is also presented. Results highlight the relevance of MHO-0730 in promoting infection and modulation and evasion of innate immunity and provide additional knowledge on M. hominis virulence and survival in the host.
2019
Cacciotto C., Dessi D., Cubeddu T., Cocco A.R., Pisano A., Tore G., et al. (2019). MHO-0730 as a Surface-Exposed Calcium-Dependent Nuclease of Mycoplasma hominis Promoting Neutrophil Extracellular Trap Formation and Escape. THE JOURNAL OF INFECTIOUS DISEASES, 220(12), 1999-2008 [10.1093/infdis/jiz406].
Cacciotto C.; Dessi D.; Cubeddu T.; Cocco A.R.; Pisano A.; Tore G.; Fiori P.L.; Rappelli P.; Pittau M.; Alberti A.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/836080
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