The authors were requested by a breeder to ascertain paternity in a litter of boxer puppies. Three different putative sires were included in parentage test although only one stud dog was seen mated with the dam. DNA was extracted either from blood and from hairs of dogs. Ten microsatellites (STRs) were chosen among those validated in dogs. Microsatellites were amplified by PCR using primers pairs reported in literature. In one case primers were also designed based on STRs flanking nucleotides sequences published on Genebank. Nine out of ten STRs were adequately amplified. Amplicons were visualised using 6% polyacrilamide gels. In 3 cases the observed migration pattern was entirely consistent with dog n° 1 being the father of puppy n°1, 2 and 3. For puppy n° 4 and 5 the parentage test was not resolved.
Gentilini F, Turba M.E., Andreani G. (2004). DNA fingerprinting using microsatellites to solve a parentage testing in the boxer breed.
DNA fingerprinting using microsatellites to solve a parentage testing in the boxer breed
GENTILINI, FABIO;TURBA, MARIA ELENA;ANDREANI, GIULIA
2004
Abstract
The authors were requested by a breeder to ascertain paternity in a litter of boxer puppies. Three different putative sires were included in parentage test although only one stud dog was seen mated with the dam. DNA was extracted either from blood and from hairs of dogs. Ten microsatellites (STRs) were chosen among those validated in dogs. Microsatellites were amplified by PCR using primers pairs reported in literature. In one case primers were also designed based on STRs flanking nucleotides sequences published on Genebank. Nine out of ten STRs were adequately amplified. Amplicons were visualised using 6% polyacrilamide gels. In 3 cases the observed migration pattern was entirely consistent with dog n° 1 being the father of puppy n°1, 2 and 3. For puppy n° 4 and 5 the parentage test was not resolved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
