The availability of portable analytical devices for on-site monitoring and rapid detection of analytes of forensic, environmental, and clinical interest is vital. We report the development of a portable device for the detection of biochemiluminescence relying on silicon photomultiplier (SiPM) technology, called LuminoSiPM, which includes a 3D printed sample holder that can be adapted for both liquid samples and paper-based biosensing. We performed a comparison of analytical performance in terms of detectability with a benchtop luminometer, a portable cooled charge-coupled device (CCD sensor), and smartphone-integrated complementary metal oxide semiconductor (CMOS) sensors. As model systems, we used two luciferase/luciferin systems emitting at different wavelengths using purified protein solutions: the green-emitting P. pyralis mutant Ppy-GR-TS (λmax 550 nm) and the blue-emitting NanoLuc (λmax 460 nm). A limit of detection of 9 femtomoles was obtained for NanoLuc luciferase, about 2 and 3 orders of magnitude lower than that obtained with the portable CCD camera and with the smartphone, respectively. A proof-of-principle forensic application of LuminoSiPM is provided, exploiting an origami chemiluminescent paper-based sensor for acetylcholinesterase inhibitors, showing high potential for this portable low-cost device for on-site applications with adequate sensitivity for detecting low light intensities in critical fields.

Ultrasensitive On-Field Luminescence Detection Using a Low-Cost Silicon Photomultiplier Device / Calabretta M.M.; Montali L.; Lopreside A.; Fragapane F.; Iacoangeli F.; Roda A.; Bocci V.; D'Elia M.; Michelini E.. - In: ANALYTICAL CHEMISTRY. - ISSN 0003-2700. - ELETTRONICO. - 93:20(2021), pp. 7388-7393. [10.1021/acs.analchem.1c00899]

Ultrasensitive On-Field Luminescence Detection Using a Low-Cost Silicon Photomultiplier Device

Calabretta M. M.
Co-primo
;
Montali L.
Co-primo
;
Lopreside A.;Roda A.;D'Elia M.
;
Michelini E.
Ultimo
2021

Abstract

The availability of portable analytical devices for on-site monitoring and rapid detection of analytes of forensic, environmental, and clinical interest is vital. We report the development of a portable device for the detection of biochemiluminescence relying on silicon photomultiplier (SiPM) technology, called LuminoSiPM, which includes a 3D printed sample holder that can be adapted for both liquid samples and paper-based biosensing. We performed a comparison of analytical performance in terms of detectability with a benchtop luminometer, a portable cooled charge-coupled device (CCD sensor), and smartphone-integrated complementary metal oxide semiconductor (CMOS) sensors. As model systems, we used two luciferase/luciferin systems emitting at different wavelengths using purified protein solutions: the green-emitting P. pyralis mutant Ppy-GR-TS (λmax 550 nm) and the blue-emitting NanoLuc (λmax 460 nm). A limit of detection of 9 femtomoles was obtained for NanoLuc luciferase, about 2 and 3 orders of magnitude lower than that obtained with the portable CCD camera and with the smartphone, respectively. A proof-of-principle forensic application of LuminoSiPM is provided, exploiting an origami chemiluminescent paper-based sensor for acetylcholinesterase inhibitors, showing high potential for this portable low-cost device for on-site applications with adequate sensitivity for detecting low light intensities in critical fields.
2021
Ultrasensitive On-Field Luminescence Detection Using a Low-Cost Silicon Photomultiplier Device / Calabretta M.M.; Montali L.; Lopreside A.; Fragapane F.; Iacoangeli F.; Roda A.; Bocci V.; D'Elia M.; Michelini E.. - In: ANALYTICAL CHEMISTRY. - ISSN 0003-2700. - ELETTRONICO. - 93:20(2021), pp. 7388-7393. [10.1021/acs.analchem.1c00899]
Calabretta M.M.; Montali L.; Lopreside A.; Fragapane F.; Iacoangeli F.; Roda A.; Bocci V.; D'Elia M.; Michelini E.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/833115
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