Endothelin (ET)-1 is a potent vasoconstrictor peptide involved in the derangement of respiratory mechanics during endotoxic shock.We measured the kinetics of pulmonary mRNA expression of the key components of the ET system [i.e., ET-1, ET-converting enzyme (ECE), and ETA and ETB receptors] by quantitative real-time reverse transcriptase-polymerase chain reaction in a swine model of endotoxic shock (0, 1, 2, 3, and 4 h of continuous LPS infusion at 40 μg/kg/hour; sham group, 4 hour saline infusion).A significant increase inmRNAexpression levels was observed for ET-1 in LPS-treated piglets; the increase began as early as 1 hour. In contrast, no significant variations were observed for the ECE, ETA, or ETB genes. Small gene expression differences observed with respect to our previous results suggest a possible effect of the anesthesia or surgical protocol on ET system regulation.
Pulmonar kinetic expression of the endothelin system in a swine model of endotoxic shock / Zannoni A; Bernardini C; Gentilini F; Giunti M; Bacci ML; Forni M.. - In: VETERINARY RESEARCH COMMUNICATIONS. - ISSN 0165-7380. - STAMPA. - 34 (Supp. 1):(2010), pp. S21-S24. (Intervento presentato al convegno LXIII ANNUAL MEETING OF THE ITALIAN SOCIETY FOR VETERINARY SCIENCES (SISVET) tenutosi a Udine nel 2009).
Pulmonar kinetic expression of the endothelin system in a swine model of endotoxic shock.
ZANNONI, AUGUSTA;BERNARDINI, CHIARA;GENTILINI, FABIO;GIUNTI, MASSIMO;BACCI, MARIA LAURA;FORNI, MONICA
2010
Abstract
Endothelin (ET)-1 is a potent vasoconstrictor peptide involved in the derangement of respiratory mechanics during endotoxic shock.We measured the kinetics of pulmonary mRNA expression of the key components of the ET system [i.e., ET-1, ET-converting enzyme (ECE), and ETA and ETB receptors] by quantitative real-time reverse transcriptase-polymerase chain reaction in a swine model of endotoxic shock (0, 1, 2, 3, and 4 h of continuous LPS infusion at 40 μg/kg/hour; sham group, 4 hour saline infusion).A significant increase inmRNAexpression levels was observed for ET-1 in LPS-treated piglets; the increase began as early as 1 hour. In contrast, no significant variations were observed for the ECE, ETA, or ETB genes. Small gene expression differences observed with respect to our previous results suggest a possible effect of the anesthesia or surgical protocol on ET system regulation.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.