Endothelin (ET)-1 is a potent vasoconstrictor peptide involved in the derangement of respiratory mechanic during endotoxic shock. We measured the kinetic of pulmonary mRNA expression of the key components of the ET system (ET-1, ET-converting enzyme (ECE), ETA and ETB receptors) by Quantitative Real Time RT-PCR in a swine model of endotoxic shock (0, 1, 2, 3, 4 h of continuous LPS infusion - 40μg/Kg/hour; Sham group, 4 h saline infusion). Significative increase of expression levels were observed for ET-1 in LPS treated piglets; the increase started as early as 1 h. On the contrary no significant variations were observed for ECE, ETA and ETB genes. Small gene expression differences observed in respect to our previous results suggest a possible effect of the anesthesiological/surgical protocol on the ET system regulation.

Cinetica dell’espressione polmonare del sistema endotelina nel modello suino di shock endotossico.

ZANNONI, AUGUSTA;BERNARDINI, CHIARA;GENTILINI, FABIO;GIUNTI, MASSIMO;BACCI, MARIA LAURA;FORNI, MONICA
2009

Abstract

Endothelin (ET)-1 is a potent vasoconstrictor peptide involved in the derangement of respiratory mechanic during endotoxic shock. We measured the kinetic of pulmonary mRNA expression of the key components of the ET system (ET-1, ET-converting enzyme (ECE), ETA and ETB receptors) by Quantitative Real Time RT-PCR in a swine model of endotoxic shock (0, 1, 2, 3, 4 h of continuous LPS infusion - 40μg/Kg/hour; Sham group, 4 h saline infusion). Significative increase of expression levels were observed for ET-1 in LPS treated piglets; the increase started as early as 1 h. On the contrary no significant variations were observed for ECE, ETA and ETB genes. Small gene expression differences observed in respect to our previous results suggest a possible effect of the anesthesiological/surgical protocol on the ET system regulation.
Atti della Società Italiana delle Scienze Veterinarie
61
62
Zannoni A; Bernardini C; Gentilini F; Giunti M; Bacci ML; Forni M.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/82312
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