CRIS Current Research Information System
IRIS è la soluzione IT che facilita la raccolta e la gestione dei dati relativi alle attività e ai prodotti della ricerca. Fornisce a ricercatori, amministratori e valutatori gli strumenti per monitorare i risultati della ricerca, aumentarne la visibilità e allocare in modo efficace le risorse disponibili.
EnglishIn milk cells from 50 healthy Holstein cows, expression levels of tumor necrosis factor-alpha (TNF-a), interleukin-8 (IL-8), and interleukin-10 (IL-10) were investigated by Real-Time TaqMan® polymerase chain reaction (PCR) systems. Samples from early- and late-lactation stages tended to express lower levels of TNF-a and IL-8 than did those from mid-lactation. On the contrary, the IL-10 expression level in samples from early- and late-lactation tended to be higher than in mid-lactation samples. Cytokines transcription in late-lactation was more co-ordinated in their transcriptional activity. The results suggest that the lactation stage-dependent changes of the quantitative cytokine mRNA profiles are crucial for monitoring the well-being of the mammary gland.
| Titolo: | EVALUATION OF TNF-a, IL-8 AND IL-10 TRANSCRIPTIONAL ACTIVITY IN MILK FROM HEALTHY DAIRY COWS DURING LACTATION PERIOD |
| Autore/i: | D. Britti; PELI, ANGELO; G. Massimini; A. Polci; A. Luciani; FAMIGLI BERGAMINI, PAOLO |
| Autore/i Unibo: | |
| Anno: | 2005 |
| Rivista: | |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1007/s11259-005-0062-9 |
| Abstract: | In milk cells from 50 healthy Holstein cows, expression levels of tumor necrosis factor-alpha (TNF-a), interleukin-8 (IL-8), and interleukin-10 (IL-10) were investigated by Real-Time TaqMan® polymerase chain reaction (PCR) systems. Samples from early- and late-lactation stages tended to express lower levels of TNF-a and IL-8 than did those from mid-lactation. On the contrary, the IL-10 expression level in samples from early- and late-lactation tended to be higher than in mid-lactation samples. Cytokines transcription in late-lactation was more co-ordinated in their transcriptional activity. The results suggest that the lactation stage-dependent changes of the quantitative cytokine mRNA profiles are crucial for monitoring the well-being of the mammary gland. |
| Data prodotto definitivo in UGOV: | 2005-10-11 17:38:33 |
| Appare nelle tipologie: | 1.01 Articolo in rivista |
File in questo prodotto:
http://hdl.handle.net/11585/8172
Copyright © 2021