EVALUATION OF TNF-a, IL-8 AND IL-10 TRANSCRIPTIONAL ACTIVITY IN MILK FROM HEALTHY DAIRY COWS DURING LACTATION PERIOD

Britti, D.; Peli, Angelo; Massimini, G.; Polci, A.; Luciani, A.; FAMIGLI BERGAMINI, Paolo

doi:10.1007/s11259-005-0062-9

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In milk cells from 50 healthy Holstein cows, expression levels of tumor necrosis factor-alpha (TNF-a), interleukin-8 (IL-8), and interleukin-10 (IL-10) were investigated by Real-Time TaqMan® polymerase chain reaction (PCR) systems. Samples from early- and late-lactation stages tended to express lower levels of TNF-a and IL-8 than did those from mid-lactation. On the contrary, the IL-10 expression level in samples from early- and late-lactation tended to be higher than in mid-lactation samples. Cytokines transcription in late-lactation was more co-ordinated in their transcriptional activity. The results suggest that the lactation stage-dependent changes of the quantitative cytokine mRNA profiles are crucial for monitoring the well-being of the mammary gland.

Titolo:	EVALUATION OF TNF-a, IL-8 AND IL-10 TRANSCRIPTIONAL ACTIVITY IN MILK FROM HEALTHY DAIRY COWS DURING LACTATION PERIOD
Autore/i:	D. Britti; PELI, ANGELO; G. Massimini; A. Polci; A. Luciani; FAMIGLI BERGAMINI, PAOLO
Autore/i Unibo:	D. Britti PELI, ANGELO G. Massimini A. Polci A. Luciani FAMIGLI BERGAMINI, PAOLO mostra contributor esterni nascondi contributor esterni
Anno:	2005
Rivista:	VETERINARY RESEARCH COMMUNICATIONS
Digital Object Identifier (DOI):	10.1007/s11259-005-0062-9
Abstract:	In milk cells from 50 healthy Holstein cows, expression levels of tumor necrosis factor-alpha (TNF-a), interleukin-8 (IL-8), and interleukin-10 (IL-10) were investigated by Real-Time TaqMan® polymerase chain reaction (PCR) systems. Samples from early- and late-lactation stages tended to express lower levels of TNF-a and IL-8 than did those from mid-lactation. On the contrary, the IL-10 expression level in samples from early- and late-lactation tended to be higher than in mid-lactation samples. Cytokines transcription in late-lactation was more co-ordinated in their transcriptional activity. The results suggest that the lactation stage-dependent changes of the quantitative cytokine mRNA profiles are crucial for monitoring the well-being of the mammary gland.
Data prodotto definitivo in UGOV:	2005-10-11 17:38:33
Appare nelle tipologie:	1.01 Articolo in rivista

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http://hdl.handle.net/11585/8172

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