The orf virus is the causal agent of contagious ecthyma; a pustular dermatitis of sheep, goats and humans. The orf is an epitheliotropic virus causing lesions, localized in skin and mucosae, that typically progress through the stages of erythema, papule, pustule and scab. Organotypic human skin cultures (rafts) have already been successfully been used in several research applications (Snoeck et al., 2002) demostrating that they can reproduce the morphology of human skin in vivo. In veterinary medicine, the organotypic skin equivalent could represent an alternative to animal models in the study of the pathogenesis of epitheliotropic viruses. The aim of this study was to develop ovine skin equivalents from differentiated cultures of lamb keratinocytes for the ex vivo study of orf virus infection.

Ovine skin organotypic cultures applied to the ex vivo study of orf virus infection

SCAGLIARINI, ALESSANDRA;DAL POZZO, FABIANA;GALLINA, LAURA;
2005

Abstract

The orf virus is the causal agent of contagious ecthyma; a pustular dermatitis of sheep, goats and humans. The orf is an epitheliotropic virus causing lesions, localized in skin and mucosae, that typically progress through the stages of erythema, papule, pustule and scab. Organotypic human skin cultures (rafts) have already been successfully been used in several research applications (Snoeck et al., 2002) demostrating that they can reproduce the morphology of human skin in vivo. In veterinary medicine, the organotypic skin equivalent could represent an alternative to animal models in the study of the pathogenesis of epitheliotropic viruses. The aim of this study was to develop ovine skin equivalents from differentiated cultures of lamb keratinocytes for the ex vivo study of orf virus infection.
Scagliarini A. ; Dal Pozzo F. ; Gallina L. ; Guercio A. ; De Clercq E. ; Snoeck R. ; Andrei G.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/8161
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