SUMMARY - Immunohistochemistry (IHC) is a valuable technique for both diagnosis and research of infectious and neoplastic diseases in a variety of animals. When dealing with fish tissues these challenges are far more arduous, because of compelling differences between mammal and fish tissues, since cross-reactivity has not been extensively tested in these animals. The aim of this research was to test a standardized panel of commercial antibodies used in our histopathology laboratory, to verify their cross-reactivity, if any, when compared with mammal tissues. Twelve commercial species were sampled. Various tissues were formalin-fixed and paraffinembedded. A tissue array was constructed for each fish; replicate sections were cut at 3 μm and underwent to a IHC protocol. The antibodies employed were directed against epithelial (AE1/AE3 cytokeratins, CK5/6, CK14, CK19, E-cadherin), mesenchymal (vimentin, desmin, α-actin, factor VIII-RA), haemopoietic (CD3, CD18 canine and CD18 feline, CD45-RA, CD79α, CD117, MAC 387), endocrine (chromogranin A, synaptophysin), cell cycle antigens (Ki67 and hTERT) and S100 protein. Seven out of 21 antibodies tested (CKAE1/AE3, CK5/6, Ecadherin, vimentin, CD117, synaptophysin, hTERT) were immunoreactive, and immunopositivity had the expected location. Our findings allow, when cross-reactivity is validated, to extend IHC application on fish species, in order to identify tissue and cell components.

R. Sirri, L. Mandrioli, M. Morini, G. Bettini (2008). Validazione della reattività immunoistochimica di un pannello anticorpale in diverse specie ittiche mediante l’impiego di “Tissue Array”. ITTIOPATOLOGIA, 5, 187-194.

Validazione della reattività immunoistochimica di un pannello anticorpale in diverse specie ittiche mediante l’impiego di “Tissue Array”

SIRRI, RUBINA;MANDRIOLI, LUCIANA;MORINI, MARIA;BETTINI, GIULIANO
2008

Abstract

SUMMARY - Immunohistochemistry (IHC) is a valuable technique for both diagnosis and research of infectious and neoplastic diseases in a variety of animals. When dealing with fish tissues these challenges are far more arduous, because of compelling differences between mammal and fish tissues, since cross-reactivity has not been extensively tested in these animals. The aim of this research was to test a standardized panel of commercial antibodies used in our histopathology laboratory, to verify their cross-reactivity, if any, when compared with mammal tissues. Twelve commercial species were sampled. Various tissues were formalin-fixed and paraffinembedded. A tissue array was constructed for each fish; replicate sections were cut at 3 μm and underwent to a IHC protocol. The antibodies employed were directed against epithelial (AE1/AE3 cytokeratins, CK5/6, CK14, CK19, E-cadherin), mesenchymal (vimentin, desmin, α-actin, factor VIII-RA), haemopoietic (CD3, CD18 canine and CD18 feline, CD45-RA, CD79α, CD117, MAC 387), endocrine (chromogranin A, synaptophysin), cell cycle antigens (Ki67 and hTERT) and S100 protein. Seven out of 21 antibodies tested (CKAE1/AE3, CK5/6, Ecadherin, vimentin, CD117, synaptophysin, hTERT) were immunoreactive, and immunopositivity had the expected location. Our findings allow, when cross-reactivity is validated, to extend IHC application on fish species, in order to identify tissue and cell components.
2008
R. Sirri, L. Mandrioli, M. Morini, G. Bettini (2008). Validazione della reattività immunoistochimica di un pannello anticorpale in diverse specie ittiche mediante l’impiego di “Tissue Array”. ITTIOPATOLOGIA, 5, 187-194.
R. Sirri; L. Mandrioli; M. Morini; G. Bettini
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/80700
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