The full-length F1L gene was amplified by PCR and cloned into pCR T7/CT-TOPO vector fused with a six-histidine tag at the C terminus. Full-length F1L protein was expressed in Escherichia coli BL21 and was purified using a metal-chelate affinity chromatography. Two rabbits were immunized with the purified recombinant full-length F1L protein in Gerbu adjuvant and the immune response of the animals was checked by Western immunoblot analysis and virus neutralization. The results showed that F1L protein is able to stimulate the production of neutralizing antibodies .
Gallina L, Scagliarini A, Ciulli S, Prosperi S. (2004). Cloning and expression of the Orf virus F1L gene: possible use as a subunit vaccine. VETERINARY RESEARCH COMMUNICATIONS, Aug;28 Suppl 1, 291-293 [10.1023/B:VERC.0000045429.78312.f6].
Cloning and expression of the Orf virus F1L gene: possible use as a subunit vaccine.
GALLINA, LAURA;SCAGLIARINI, ALESSANDRA;CIULLI, SARA;PROSPERI, SANTINO
2004
Abstract
The full-length F1L gene was amplified by PCR and cloned into pCR T7/CT-TOPO vector fused with a six-histidine tag at the C terminus. Full-length F1L protein was expressed in Escherichia coli BL21 and was purified using a metal-chelate affinity chromatography. Two rabbits were immunized with the purified recombinant full-length F1L protein in Gerbu adjuvant and the immune response of the animals was checked by Western immunoblot analysis and virus neutralization. The results showed that F1L protein is able to stimulate the production of neutralizing antibodies .I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
