Cloning and expression of allergens in apple fruits

Apple, the most common fruit in European and North American diets, can cause allergic reactions in susceptible individuals, and genes for four families of apple allergens have been identified to date: Mal d 1, Mal d 2, Mal d 3, and Mal d 4. Our remit was to evaluate the effects of genotype, tissue, and stage of fruit development on the expression of these allergen genes and, hence, on the potential allergenicity of apple fruit.Transcript levels were investigated in fruit of the apple cultivars, ‘Gala’ and ‘Florina’, using quantitative Real-Time PCR.The resulting patterns of allergen gene expression differed, with Mal d 1 and Mal d 2 being the most highly expressed in the skin and flesh of ripe fruit, respectively. Overall, ‘Florina’ fruit showed higher levels of expression than ‘Gala’ fruit for all allergens tested.