Confocal laser scanning microscopy provides nondestructive in vivo imaging to capture specific structures that have been fluorescently labeled, such as cell nuclei and membranes, throughout early Zebrafish embryogenesis. With this strategy we aim at reconstruct in time and space the biological structures of the embryo during the organogenesis. In this paper we propose a method to extract bounding surfaces at the cellular-organization level from microscopy images. The shape reconstruction of membranes and nuclei is obtained first with an automatic identification of the cell center and then a subjective surfaces based segmentation is used to extract the bounding surfaces.

A Subjective Surfaces Based Segmentation for the Reconstruction of Biological Cell Shape

CAMPANA, MATTEO;ZANELLA, CECILIA;RIZZI, BARBARA;MELANI, CAMILO;SARTI, ALESSANDRO
2008

Abstract

Confocal laser scanning microscopy provides nondestructive in vivo imaging to capture specific structures that have been fluorescently labeled, such as cell nuclei and membranes, throughout early Zebrafish embryogenesis. With this strategy we aim at reconstruct in time and space the biological structures of the embryo during the organogenesis. In this paper we propose a method to extract bounding surfaces at the cellular-organization level from microscopy images. The shape reconstruction of membranes and nuclei is obtained first with an automatic identification of the cell center and then a subjective surfaces based segmentation is used to extract the bounding surfaces.
Proceedings of the Third International Conference on Computer Vision Theory and Applications
555
558
M. Campana; C. Zanella; B. Rizzi; C. Melani; P. Bourgine; N.Peyriéras; A. Sarti
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11585/79788
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