Nicotine addiction is a severe public health problem. The aim of this study was to investigate the alterations in key neurotransmissions after 60 days of withdrawal from seven weeks of intermittent cigarette smoke, e-cigarette vapours, or an e-cigarette vehicle. In the nicotine withdrawal groups, increased depressive and anxiety/obsessive–compulsive-like behaviours were demonstrated in the tail suspension, sucrose preference and marble burying tests. Cognitive impairments were detected in the spatial object recognition test. A significant increase in Corticotropin-releasing factor (Crf) and Crf1 mRNA levels was observed, specifically after cigarette withdrawal in the caudate-putamen nucleus (CPu). The nociceptin precursor levels were reduced by cigarette (80%) and e-cigarette (50%) withdrawal in the CPu. The delta opioid receptor showed a significant reduction in the hippocampus driven by the exposure to an e-cigarette solubilisation vehicle, while the mRNA levels doubled in the CPu of mice that had been exposed to e-cigarettes. Withdrawal after exposure to e-cigarette vapour induced a 35% Bdnf mRNA decrease in the hippocampus, whereas Bdnf was augmented by 118% by cigarette withdrawal in the CPu. This study shows that long-term withdrawal-induced affective and cognitive symptoms associated to lasting molecular alterations in peptidergic signalling may determine the impaired neuroplasticity in the hippocampal and striatal circuitry.

Carboni L., Ponzoni L., Braida D., Sala M., Gotti C., Zoli M. (2021). Altered mrna levels of stress-related peptides in mouse hippocampus and caudate-putamen in withdrawal after long-term intermittent exposure to tobacco smoke or electronic cigarette vapour. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 22(2), 1-17 [10.3390/ijms22020599].

Altered mrna levels of stress-related peptides in mouse hippocampus and caudate-putamen in withdrawal after long-term intermittent exposure to tobacco smoke or electronic cigarette vapour

Carboni L.;
2021

Abstract

Nicotine addiction is a severe public health problem. The aim of this study was to investigate the alterations in key neurotransmissions after 60 days of withdrawal from seven weeks of intermittent cigarette smoke, e-cigarette vapours, or an e-cigarette vehicle. In the nicotine withdrawal groups, increased depressive and anxiety/obsessive–compulsive-like behaviours were demonstrated in the tail suspension, sucrose preference and marble burying tests. Cognitive impairments were detected in the spatial object recognition test. A significant increase in Corticotropin-releasing factor (Crf) and Crf1 mRNA levels was observed, specifically after cigarette withdrawal in the caudate-putamen nucleus (CPu). The nociceptin precursor levels were reduced by cigarette (80%) and e-cigarette (50%) withdrawal in the CPu. The delta opioid receptor showed a significant reduction in the hippocampus driven by the exposure to an e-cigarette solubilisation vehicle, while the mRNA levels doubled in the CPu of mice that had been exposed to e-cigarettes. Withdrawal after exposure to e-cigarette vapour induced a 35% Bdnf mRNA decrease in the hippocampus, whereas Bdnf was augmented by 118% by cigarette withdrawal in the CPu. This study shows that long-term withdrawal-induced affective and cognitive symptoms associated to lasting molecular alterations in peptidergic signalling may determine the impaired neuroplasticity in the hippocampal and striatal circuitry.
2021
Carboni L., Ponzoni L., Braida D., Sala M., Gotti C., Zoli M. (2021). Altered mrna levels of stress-related peptides in mouse hippocampus and caudate-putamen in withdrawal after long-term intermittent exposure to tobacco smoke or electronic cigarette vapour. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 22(2), 1-17 [10.3390/ijms22020599].
Carboni L.; Ponzoni L.; Braida D.; Sala M.; Gotti C.; Zoli M.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/792445
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