Discerning with crop health issues, this study was conducted to detect and identify phytoplasmas and their potential insect vectors in symptomatic Fenugreek (Trigonella foenum-graecum) plants collected from Punjab, Pakistan. The detection of phytoplasma in collected leafhopper species and Fenugreek plants was confirmed by nested PCR amplification of 16SrDNA by employing primer pairs (P1/P7 & R16F2n/R16R2). Our results indicated that all the symptomatic fenugreek plant and specimens of leafhoppers, Orosius albicinctus, Empoasca spp., and Balclutha incisa resulted positive in PCR. Sequencing of amplified DNA products and phylogenetic analysis of our Fenugreek phyllody phytoplasma (Accession number MH398586) showed that the phytoplasma strains detected has maximum identity (100%) with ‘Candidatus Phytoplasma australasiae’ subgroup 16Sr-II-D (Gen Bank number Y10097. This is the first detection and identification of phytoplasma presence in fenugreek seed plants with putative potential insect vectors in Faisalabad, Punjab, Pakistan.
Malik S.T., Ahmad J.N., Sharif M.Z., Trebicki P., Tahir M., Bertaccini A. (2020). Molecular detection and characterisation of phytoplasma in Trigonella foenum-graecum and identification of potential insect vectors in Punjab, Pakistan. PAKISTAN JOURNAL OF BOTANY, 52(5), 1605-1613 [10.30848/PJB2020-5(16)].
Molecular detection and characterisation of phytoplasma in Trigonella foenum-graecum and identification of potential insect vectors in Punjab, Pakistan
Bertaccini A.
2020
Abstract
Discerning with crop health issues, this study was conducted to detect and identify phytoplasmas and their potential insect vectors in symptomatic Fenugreek (Trigonella foenum-graecum) plants collected from Punjab, Pakistan. The detection of phytoplasma in collected leafhopper species and Fenugreek plants was confirmed by nested PCR amplification of 16SrDNA by employing primer pairs (P1/P7 & R16F2n/R16R2). Our results indicated that all the symptomatic fenugreek plant and specimens of leafhoppers, Orosius albicinctus, Empoasca spp., and Balclutha incisa resulted positive in PCR. Sequencing of amplified DNA products and phylogenetic analysis of our Fenugreek phyllody phytoplasma (Accession number MH398586) showed that the phytoplasma strains detected has maximum identity (100%) with ‘Candidatus Phytoplasma australasiae’ subgroup 16Sr-II-D (Gen Bank number Y10097. This is the first detection and identification of phytoplasma presence in fenugreek seed plants with putative potential insect vectors in Faisalabad, Punjab, Pakistan.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
