We devised a novel Western blot (WB) test for anti-human cytomegalovirus (HCMV) immunoglobulin M (IgM) detection which contains viral structural polypeptides, significant portions of recombinant p150 (ppUL32), and a significant portion of the must immunogenic nonstructural protein p52 (ppUL44). This new test was evaluated in latently infected blood donors, pregnant women, and transplant recipients with ongoing HCMV infection and shown to be more sensitive and specific than traditional WB and conventional enzyme immunoassay for the detection of HCMV-specific IgM.
A novel Western blot test containing both viral and recombinant proteins for anticytomegalovirus immunoglobulin M detection / Lazzarotto T, Maine GT, Dal Monte P, Ripalti A, Landini MP. - In: JOURNAL OF CLINICAL MICROBIOLOGY. - ISSN 1098-660X. - STAMPA. - 35:2(1997), pp. 393-397. [10.1128/JCM.35.2.393-397.1997]
A novel Western blot test containing both viral and recombinant proteins for anticytomegalovirus immunoglobulin M detection.
Lazzarotto T;Dal Monte P;Landini MP
1997
Abstract
We devised a novel Western blot (WB) test for anti-human cytomegalovirus (HCMV) immunoglobulin M (IgM) detection which contains viral structural polypeptides, significant portions of recombinant p150 (ppUL32), and a significant portion of the must immunogenic nonstructural protein p52 (ppUL44). This new test was evaluated in latently infected blood donors, pregnant women, and transplant recipients with ongoing HCMV infection and shown to be more sensitive and specific than traditional WB and conventional enzyme immunoassay for the detection of HCMV-specific IgM.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
