Surface-Initiated Atom Transfer Radical Polymerization (SI-ATRP) is a promising tool for conferring specific physico-chemical and biological properties to surfaces. Here we have employed SI-ATRP to functionalize substrates for cell culture. A cationic macroinitiator was adsorbed on oxidized Tissue Culture Polystyrene (TCPS) surfaces to allow the growth of surface-born polymer chains, producing films with controlled thickness. We have then focused our attention on the preparation of films containing poly(glycerol monomethacrylate) (PGMMA), showing that PGMMA is non-toxic but non-adhesive to cells, possibly providing “stealth” surfaces. Cell adhesion can, however, be provided through copolymerization with appropriate monomers: films containing N,N-dimethylamino ethyl methacrylate (DMAEMA: substantially hydrophobic at physiological pH) together with GMMA provided cell attachment and spreading to comparable to TCPS
E. Patrucco, S. Ouasti, C. D. Vo, P. De Leonardis, A. Pollicino, S. P. Armes, et al. (2009). Surface-ATRP (SI-ATRP) modification of tissue culture substrates. Poly(glycerol methacrylate) as an anti-fouling surface. BIOMACROMOLECULES, 10, 3130-3140 [10.1021/bm900856r].
Surface-ATRP (SI-ATRP) modification of tissue culture substrates. Poly(glycerol methacrylate) as an anti-fouling surface
SCANDOLA, MARIASTELLA;
2009
Abstract
Surface-Initiated Atom Transfer Radical Polymerization (SI-ATRP) is a promising tool for conferring specific physico-chemical and biological properties to surfaces. Here we have employed SI-ATRP to functionalize substrates for cell culture. A cationic macroinitiator was adsorbed on oxidized Tissue Culture Polystyrene (TCPS) surfaces to allow the growth of surface-born polymer chains, producing films with controlled thickness. We have then focused our attention on the preparation of films containing poly(glycerol monomethacrylate) (PGMMA), showing that PGMMA is non-toxic but non-adhesive to cells, possibly providing “stealth” surfaces. Cell adhesion can, however, be provided through copolymerization with appropriate monomers: films containing N,N-dimethylamino ethyl methacrylate (DMAEMA: substantially hydrophobic at physiological pH) together with GMMA provided cell attachment and spreading to comparable to TCPSI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
