A method of reprogramming adult cells to iPSC is disclosed. The method comprises seeding adult cells in a microfluidic cell culture chamber; submitting the seeded cells to reprogramming by delivering into said cells suitable reprogramming factors and/or inducing into said cells the synthesis of suitable reprogramming factors, with suitable vectors and culturing said cells until iPSC are obtained. The height of the culture medium comprising said suitable reprogramming factors and/or vectors in the microfluidic cell culture chamber with respect to the bottom of the chamber is in the range from 500 to 50 µm. A microfluidic system configured to perform cell reprogramming is further disclosed.

ELVASSORE NICOLA, GAGLIANO ONELIA, GIULITTI STEFANO, LUNI CAMILLA, SERENA ELENA, ZAMBON ALESSANDRO (2017). A NEW METHOD FOR THE REPROGRAMMING OF CELLS.

A NEW METHOD FOR THE REPROGRAMMING OF CELLS

LUNI CAMILLA;ZAMBON ALESSANDRO
2017

Abstract

A method of reprogramming adult cells to iPSC is disclosed. The method comprises seeding adult cells in a microfluidic cell culture chamber; submitting the seeded cells to reprogramming by delivering into said cells suitable reprogramming factors and/or inducing into said cells the synthesis of suitable reprogramming factors, with suitable vectors and culturing said cells until iPSC are obtained. The height of the culture medium comprising said suitable reprogramming factors and/or vectors in the microfluidic cell culture chamber with respect to the bottom of the chamber is in the range from 500 to 50 µm. A microfluidic system configured to perform cell reprogramming is further disclosed.
2017
WO2017179021A1
ELVASSORE NICOLA, GAGLIANO ONELIA, GIULITTI STEFANO, LUNI CAMILLA, SERENA ELENA, ZAMBON ALESSANDRO (2017). A NEW METHOD FOR THE REPROGRAMMING OF CELLS.
ELVASSORE NICOLA; GAGLIANO ONELIA; GIULITTI STEFANO; LUNI CAMILLA; SERENA ELENA; ZAMBON ALESSANDRO
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/776348
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