A method of reprogramming adult cells to iPSC is disclosed. The method comprises seeding adult cells in a microfluidic cell culture chamber; submitting the seeded cells to reprogramming by delivering into said cells suitable reprogramming factors and/or inducing into said cells the synthesis of suitable reprogramming factors, with suitable vectors and culturing said cells until iPSC are obtained. The height of the culture medium comprising said suitable reprogramming factors and/or vectors in the microfluidic cell culture chamber with respect to the bottom of the chamber is in the range from 500 to 50 µm. A microfluidic system configured to perform cell reprogramming is further disclosed.
A NEW METHOD FOR THE REPROGRAMMING OF CELLS / ELVASSORE NICOLA; GAGLIANO ONELIA; GIULITTI STEFANO; LUNI CAMILLA; SERENA ELENA; ZAMBON ALESSANDRO. - (2017).
A NEW METHOD FOR THE REPROGRAMMING OF CELLS
LUNI CAMILLA;ZAMBON ALESSANDRO
2017
Abstract
A method of reprogramming adult cells to iPSC is disclosed. The method comprises seeding adult cells in a microfluidic cell culture chamber; submitting the seeded cells to reprogramming by delivering into said cells suitable reprogramming factors and/or inducing into said cells the synthesis of suitable reprogramming factors, with suitable vectors and culturing said cells until iPSC are obtained. The height of the culture medium comprising said suitable reprogramming factors and/or vectors in the microfluidic cell culture chamber with respect to the bottom of the chamber is in the range from 500 to 50 µm. A microfluidic system configured to perform cell reprogramming is further disclosed.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
