Microengineering human “organs-on-chips” remains an open challenge. Here, we describe a robust microfluidics-based approach for the differentiation of human pluripotent stem cells directly on a chip. Extrinsic signal modulation, achieved through optimal frequency of medium delivery, can be used as a parameter for improved germ layer specification and cell differentiation. Human cardiomyocytes and hepatocytes derived on chips showed functional phenotypes and responses to temporally defined drug treatments.
Functional differentiation of human pluripotent stem cells on a chip / Giobbe Giovanni G.; Michielin Federica; Luni Camilla; Giulitti Stefano; Martewicz Sebastian; Dupont Sirio; Floreani Annarosa; Elvassore Nicola. - In: NATURE METHODS. - ISSN 1548-7091. - STAMPA. - 12:7(2015), pp. 637-640. [10.1038/nmeth.3411]
Functional differentiation of human pluripotent stem cells on a chip
Luni Camilla;
2015
Abstract
Microengineering human “organs-on-chips” remains an open challenge. Here, we describe a robust microfluidics-based approach for the differentiation of human pluripotent stem cells directly on a chip. Extrinsic signal modulation, achieved through optimal frequency of medium delivery, can be used as a parameter for improved germ layer specification and cell differentiation. Human cardiomyocytes and hepatocytes derived on chips showed functional phenotypes and responses to temporally defined drug treatments.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
