Petroleum pollution is a global concern because it causes serious environmental and human health problems. Microbial communities play important roles in petroleum biodegradation. In this study, a synthetic bacterial consortium for efficiently degrading ability was constructed on the basis of a minimal value algorithm running on MATLAB. Functional microbial diversity-based screening of three petroleum-degrading strains, Achromobacter sp. P3, Sphingobium sp. P10 and Rhizobium sp. P14, enabled the initial synthesis of the bacterial consortium in combination with a previously used bacterial consortium PDM. When 27.0% of P3, 21.0% of P10, 28.0% of P14 and 24.0% of consortium PDM were inoculated to a petroleum medium, 78.9% of petroleum was degraded under the optimal conditions. The synthetic bacterial consortium has 34.8% higher petroleum degradation than the original bacterial community PDM. Total 40% of the petroleum in the contaminated soil was removed after 20 days of soil bioremediation using the reconstructed consortium. This is an attempt to remediate petroleum-contaminated pollution with synthetic biology via reconstructing a synthetic bacterial community, providing a new idea for the remediation of other pollution problems.

Maximization of the petroleum biodegradation using a synthetic bacterial consortium based on minimal value algorithm

Zanaroli G.;
2020

Abstract

Petroleum pollution is a global concern because it causes serious environmental and human health problems. Microbial communities play important roles in petroleum biodegradation. In this study, a synthetic bacterial consortium for efficiently degrading ability was constructed on the basis of a minimal value algorithm running on MATLAB. Functional microbial diversity-based screening of three petroleum-degrading strains, Achromobacter sp. P3, Sphingobium sp. P10 and Rhizobium sp. P14, enabled the initial synthesis of the bacterial consortium in combination with a previously used bacterial consortium PDM. When 27.0% of P3, 21.0% of P10, 28.0% of P14 and 24.0% of consortium PDM were inoculated to a petroleum medium, 78.9% of petroleum was degraded under the optimal conditions. The synthetic bacterial consortium has 34.8% higher petroleum degradation than the original bacterial community PDM. Total 40% of the petroleum in the contaminated soil was removed after 20 days of soil bioremediation using the reconstructed consortium. This is an attempt to remediate petroleum-contaminated pollution with synthetic biology via reconstructing a synthetic bacterial community, providing a new idea for the remediation of other pollution problems.
2020
Cui J.; Huang L.; Wang W.; Xu P.; Zanaroli G.; Tang H.
File in questo prodotto:
Eventuali allegati, non sono esposti

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/773361
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 9
  • ???jsp.display-item.citation.isi??? 6
social impact