Testing and monitoring anabolic androgenic steroids in biological fluids is a key activity in anti-doping practices. In this study, a novel approach is proposed, based on dried urine microsampling through two different workflows: dried urine spots (DUS) and volumetric absorptive microsampling (VAMS). Both techniques can overcome some common drawbacks of urine sampling, such as analyte instability and storage and transportation problems. Using an original, validated liquid chromatography-tandem mass spectrometry (LC-MS/MS) method, exogenous and endogenous unconjugated steroids were analysed. Despite the limitations of microsampling volume, good sensitivity was obtained (limit of quantitation <1.5 ng/mL for all analytes), with satisfactory precision (relative standard deviation <7.6%) and absolute recovery (>70.3%). Both microsampling platforms provide reliable results, in good agreement with those obtained from urine.

Protti M., Marasca C., Cirrincione M., Sberna A.E., Mandrioli R., Mercolini L. (2020). Dried urine microsampling coupled to liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the analysis of unconjugated anabolic androgenic steroids. MOLECULES, 25(14), 1-18 [10.3390/molecules25143210].

Dried urine microsampling coupled to liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the analysis of unconjugated anabolic androgenic steroids

Protti M.;Marasca C.;Cirrincione M.;Mandrioli R.;Mercolini L.
2020

Abstract

Testing and monitoring anabolic androgenic steroids in biological fluids is a key activity in anti-doping practices. In this study, a novel approach is proposed, based on dried urine microsampling through two different workflows: dried urine spots (DUS) and volumetric absorptive microsampling (VAMS). Both techniques can overcome some common drawbacks of urine sampling, such as analyte instability and storage and transportation problems. Using an original, validated liquid chromatography-tandem mass spectrometry (LC-MS/MS) method, exogenous and endogenous unconjugated steroids were analysed. Despite the limitations of microsampling volume, good sensitivity was obtained (limit of quantitation <1.5 ng/mL for all analytes), with satisfactory precision (relative standard deviation <7.6%) and absolute recovery (>70.3%). Both microsampling platforms provide reliable results, in good agreement with those obtained from urine.
2020
Protti M., Marasca C., Cirrincione M., Sberna A.E., Mandrioli R., Mercolini L. (2020). Dried urine microsampling coupled to liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the analysis of unconjugated anabolic androgenic steroids. MOLECULES, 25(14), 1-18 [10.3390/molecules25143210].
Protti M.; Marasca C.; Cirrincione M.; Sberna A.E.; Mandrioli R.; Mercolini L.
File in questo prodotto:
File Dimensione Formato  
Molecules_25_2020_3210.pdf

accesso aperto

Tipo: Versione (PDF) editoriale
Licenza: Licenza per Accesso Aperto. Creative Commons Attribuzione (CCBY)
Dimensione 1.86 MB
Formato Adobe PDF
1.86 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/770421
Citazioni
  • ???jsp.display-item.citation.pmc??? 1
  • Scopus 17
  • ???jsp.display-item.citation.isi??? 13
social impact