The present work was finalised to develop chemiluminescent assays more sensitive and easy to use than the analytical methods currently in use to detect traces of TNT (2,4,6-trinitrotoluene), TATP (Triacetone triperoxide) and HMTD (Hexamethylene triperoxide diamine). For TNT detection we developed an indirect competitive ELISA with chemiluminescent end-point (CL-ELISA) and a Lateral Flow ImmunoAssay (LFIA) for on-site analysis, based both on monoclonal antibody commercially available and conjugates specifically synthesized. For the peroxide-based explosives TATP and HMTD we developed an indirect assay which measures, by luminol emission, the H2O2 released by these compounds. Both methods showed good sensitivity and reproducibility, with detection limit, LOD, and IC50 values in the ng mL-1 range, CV values lower than 10%. This indirect assay was finally optimized for using in a portable luminometer

E.Maiolini, S.Girotti, E.Ferri, P.Caputo, G.Guarnieri, S.A.Eremin, et al. (2009). Development of chemiluminescent methods for explosives detection. ANALELE UNIVERSITATII "OVIDIUS" CONSTANTA. SERIA CHIMIE, 20 (1), 57-60.

Development of chemiluminescent methods for explosives detection

MAIOLINI, ELISABETTA;GIROTTI, STEFANO;FERRI, ELIDA NORA;CAPUTO, PASQUALE;M. D’Elia
2009

Abstract

The present work was finalised to develop chemiluminescent assays more sensitive and easy to use than the analytical methods currently in use to detect traces of TNT (2,4,6-trinitrotoluene), TATP (Triacetone triperoxide) and HMTD (Hexamethylene triperoxide diamine). For TNT detection we developed an indirect competitive ELISA with chemiluminescent end-point (CL-ELISA) and a Lateral Flow ImmunoAssay (LFIA) for on-site analysis, based both on monoclonal antibody commercially available and conjugates specifically synthesized. For the peroxide-based explosives TATP and HMTD we developed an indirect assay which measures, by luminol emission, the H2O2 released by these compounds. Both methods showed good sensitivity and reproducibility, with detection limit, LOD, and IC50 values in the ng mL-1 range, CV values lower than 10%. This indirect assay was finally optimized for using in a portable luminometer
2009
E.Maiolini, S.Girotti, E.Ferri, P.Caputo, G.Guarnieri, S.A.Eremin, et al. (2009). Development of chemiluminescent methods for explosives detection. ANALELE UNIVERSITATII "OVIDIUS" CONSTANTA. SERIA CHIMIE, 20 (1), 57-60.
E.Maiolini; S.Girotti; E.Ferri; P.Caputo; G.Guarnieri; S.A.Eremin; A.Montoya; M.J.Moreno; M.D’Elia.
File in questo prodotto:
Eventuali allegati, non sono esposti

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/77015
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact