Bivalve molluscs from Adriatic sea were analyzed for V. parahaemolyticus, V. cholerae e V. vulnificus presence. The isolates on TCBS Agar and m-CPC Agar were selected on the basis of a new biochemical screening, that showed a good performance, because among 2344 strains from primary culture only 237 (10%) were presumptively assigned to the species of interest. The PCR analyses was performed for the target genes toxR hlyA, ctxA, tcpI (V. cholerae), toxR, tl, tdh, trh (V. parahaemolyticus), vvhA and viuB (V. vulnificus). Among the 9 strains confirmed to belong to V. parahaemolyticus specie, 6 were sucrose positive. On 215 samples of molluscs only 5 resulted positive for V. parahaemolyticus being toxR+, tl+, although non pathogenic (tdh-, trh-), and none for V. cholerae e V. vulnificus.
P. Serratore, A. Piano, S. Piraccini, M. Trentini, E. Zavatta, M. Grodzki, et al. (2009). Indagine sulla diffusione di V. cholerae, V. vulnificus e V. parahaemolyticus, in molluschi bivalvi dell’Adriatico e proposta di un protocollo analitico. RIVISTA DELL'ASSOCIAZIONE ITALIANA DEI VETERINARI IGIENISTI, 4, 33-38.
Indagine sulla diffusione di V. cholerae, V. vulnificus e V. parahaemolyticus, in molluschi bivalvi dell’Adriatico e proposta di un protocollo analitico
SERRATORE, PATRIZIA;PIANO, ANNAMARIA;GRODZKI, MARCO;
2009
Abstract
Bivalve molluscs from Adriatic sea were analyzed for V. parahaemolyticus, V. cholerae e V. vulnificus presence. The isolates on TCBS Agar and m-CPC Agar were selected on the basis of a new biochemical screening, that showed a good performance, because among 2344 strains from primary culture only 237 (10%) were presumptively assigned to the species of interest. The PCR analyses was performed for the target genes toxR hlyA, ctxA, tcpI (V. cholerae), toxR, tl, tdh, trh (V. parahaemolyticus), vvhA and viuB (V. vulnificus). Among the 9 strains confirmed to belong to V. parahaemolyticus specie, 6 were sucrose positive. On 215 samples of molluscs only 5 resulted positive for V. parahaemolyticus being toxR+, tl+, although non pathogenic (tdh-, trh-), and none for V. cholerae e V. vulnificus.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
