Retrotransposons constitute an abundant class of mobile genetic elements in all eukaryotic genomes. Due to their mechanism of replication via an RNA intermediate, retrotransposons are not excided from their insertion sites during the transposition and they gradually accumulate in genomes especially for plants, in which they represent the major class of repetitive DNA; moreover, it has been hypothesized that the accumulation of retrotransposon copies played an important role in growth of genome sizes. Due to their ubiquity in plants, their high copy number, and dispersion in the genome, retrotransposons provided an ideal tool for the development of molecular markers. Sequence-Specific Amplified Polymorphism (S-SAP), the first retrotransposon-based marker technique, proved to be able to distinguish different sports of apple polyclonal varieties. In the present work S-SAP markers were tested to distinguish the different sports of the European pear cultivar Bartlett. Three primer combinations, among those tested, produced polymorphic bands that distinguished ‘Max Red Bartlett’, ‘Rosired’, and ‘Sensation’ from ‘Bartlett’. The results discussed here also confirm the ability in pear to discriminate the polyclonal variety sports already described in apple.
Retrotrasposon based markers to discriminate sports in pear.
DE FRANCESCHI, PAOLO;DONDINI, LUCA;SANSAVINI, SILVIERO
2009
Abstract
Retrotransposons constitute an abundant class of mobile genetic elements in all eukaryotic genomes. Due to their mechanism of replication via an RNA intermediate, retrotransposons are not excided from their insertion sites during the transposition and they gradually accumulate in genomes especially for plants, in which they represent the major class of repetitive DNA; moreover, it has been hypothesized that the accumulation of retrotransposon copies played an important role in growth of genome sizes. Due to their ubiquity in plants, their high copy number, and dispersion in the genome, retrotransposons provided an ideal tool for the development of molecular markers. Sequence-Specific Amplified Polymorphism (S-SAP), the first retrotransposon-based marker technique, proved to be able to distinguish different sports of apple polyclonal varieties. In the present work S-SAP markers were tested to distinguish the different sports of the European pear cultivar Bartlett. Three primer combinations, among those tested, produced polymorphic bands that distinguished ‘Max Red Bartlett’, ‘Rosired’, and ‘Sensation’ from ‘Bartlett’. The results discussed here also confirm the ability in pear to discriminate the polyclonal variety sports already described in apple.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
