When high numbers of analyses are required a rapid sample preparation method coupled to RT-qPCR allows to reduce the time and the costs for the phytoplasma detection in plant samples. A comparison between DNA and RNA contribution to qPCR detection revealed the higher input of the latter ensuring the maintenance of the sensitivity and specificity of the assay. The protocol has been validated for the detection and quantification of ‘Candidatus Phytoplasma mali’, ‘Ca. P. pyri’, ‘Ca. P. prunorum’, ‘Ca. P. solani’ and “flavescence dorée” phytoplasma by qPCR, RT-qPCR, ddPCR and ddRT-PCR techniques based on TaqMan chemistry.
Phytoplasma detection and quantification: Make it easy / Ratti C.; Minguzzi S.; Lanzoni Chiara.; Poggi Pollini C.; Turina M.. - In: PHYTOPATHOGENIC MOLLICUTES. - ISSN 2249-4669. - ELETTRONICO. - 9:1(2019), pp. 83-84. [10.5958/2249-4677.2019.00042.2]
Phytoplasma detection and quantification: Make it easy
Ratti C.
;Poggi Pollini C.;
2019
Abstract
When high numbers of analyses are required a rapid sample preparation method coupled to RT-qPCR allows to reduce the time and the costs for the phytoplasma detection in plant samples. A comparison between DNA and RNA contribution to qPCR detection revealed the higher input of the latter ensuring the maintenance of the sensitivity and specificity of the assay. The protocol has been validated for the detection and quantification of ‘Candidatus Phytoplasma mali’, ‘Ca. P. pyri’, ‘Ca. P. prunorum’, ‘Ca. P. solani’ and “flavescence dorée” phytoplasma by qPCR, RT-qPCR, ddPCR and ddRT-PCR techniques based on TaqMan chemistry.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
