Due to a long life span and a high economical and emotional value koi carp (Cyprinus carpio koi) is considered as a pet worldwide [1]. In this species descriptions of gonadal tumors exist since years [2], however their occurrence only recently represents a regular finding [1]. Given the difficulty to fit into histological classification and to assess the incidence of these tumours in koi populations, the present work aims to describe and classify gonadal tumours by using an immunohistochemical tissue microarray (TMA) technique. Seventeen cases of koi gonadal tumors collected in Belgium by a veterinary practitioner from 2014 to 2015 were included. They were evaluated according to the veterinary WHO classification [3] and characterized with an antibody panel (vimentin, CD117, AE1/AE3 cytokeratin, E-cadherin, PLAP) by using TMA technique. The koi carps were held in private ponds, their age ranged between 3 and 12 years and they were mostly females. The affected fish exhibited abdominal enlargement, lethargy and anorexia. Grossly, the neoplasms were white-yellowish with soft cystic areas and multifocal haemorrhages. Twelve tumors were histologically diagnosed as sex cord-stromal tumors (SCST), three were mixed germ cell sex cord-stromal tumors (MGCSCST), two were germ cell tumors (GCT), and one was a carcinoma. AE1/AE3 cytokeratin strongly labelled the carcinoma and several SCST, but rarely GCT; E-cadherin strongly labelled almost all SCST, MGCSCST and GCT. Vimentin and CD117 were only expressed in one SCST and one MGCSCST, respectively. Our results indicate sex cord-stromal cells as the main cells of origin in accordance with a recent paper [1]. The diagnosis of gonadal tumours in fish as in mammals is still difficult due to the variety of histological patterns of SCST and the occurrence of GCT and SCST that mimic epithelial gonadal tumours [3,4]. Furthermore, none of the immunohistochemical markers can be considered specific for a tumor type and the use of a wide panel of antibodies is suggested [4,5]. Nonetheless, the antibodies here tested confirmed to be immunoreactive in fish tissues [6] and therefore useful for refining the histological diagnosis.

Gonadal tumors in koi carp (cyprinus carpio koi): immunohistochemical approach through tissue microarray technique.

SIRRI R.
Conceptualization
;
TURA G.
Membro del Collaboration Group
;
FIORENTINO M.
Membro del Collaboration Group
;
SARLI G.
Membro del Collaboration Group
;
MANDRIOLI L.
Conceptualization
2019

Abstract

Due to a long life span and a high economical and emotional value koi carp (Cyprinus carpio koi) is considered as a pet worldwide [1]. In this species descriptions of gonadal tumors exist since years [2], however their occurrence only recently represents a regular finding [1]. Given the difficulty to fit into histological classification and to assess the incidence of these tumours in koi populations, the present work aims to describe and classify gonadal tumours by using an immunohistochemical tissue microarray (TMA) technique. Seventeen cases of koi gonadal tumors collected in Belgium by a veterinary practitioner from 2014 to 2015 were included. They were evaluated according to the veterinary WHO classification [3] and characterized with an antibody panel (vimentin, CD117, AE1/AE3 cytokeratin, E-cadherin, PLAP) by using TMA technique. The koi carps were held in private ponds, their age ranged between 3 and 12 years and they were mostly females. The affected fish exhibited abdominal enlargement, lethargy and anorexia. Grossly, the neoplasms were white-yellowish with soft cystic areas and multifocal haemorrhages. Twelve tumors were histologically diagnosed as sex cord-stromal tumors (SCST), three were mixed germ cell sex cord-stromal tumors (MGCSCST), two were germ cell tumors (GCT), and one was a carcinoma. AE1/AE3 cytokeratin strongly labelled the carcinoma and several SCST, but rarely GCT; E-cadherin strongly labelled almost all SCST, MGCSCST and GCT. Vimentin and CD117 were only expressed in one SCST and one MGCSCST, respectively. Our results indicate sex cord-stromal cells as the main cells of origin in accordance with a recent paper [1]. The diagnosis of gonadal tumours in fish as in mammals is still difficult due to the variety of histological patterns of SCST and the occurrence of GCT and SCST that mimic epithelial gonadal tumours [3,4]. Furthermore, none of the immunohistochemical markers can be considered specific for a tumor type and the use of a wide panel of antibodies is suggested [4,5]. Nonetheless, the antibodies here tested confirmed to be immunoreactive in fish tissues [6] and therefore useful for refining the histological diagnosis.
73° Convegno SISVET - XVI Convegno AIPVET
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SIRRI R., TURA G., BUDAI J., BERALDO P., FIORENTINO M., BARBÉ T., GALEOTTI M., SARLI G., MANDRIOLI L.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11585/725573
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