Affinity membrane chromatography is one of the processes considered as an alternative to conventional Protein A bead-based chromatography for the primary capture step of a monoclonal antibody production process. In this work, new affinity membranes endowed with a very interesting binding capacity for human IgG have been experimentally characterized. The membranes have been extensively tested in dynamic experiments with pure IgG solutions and with a cell culture supernatant containing IgG1. The effects of flow rate and IgG concentration in the feed on the separation performances like binding capacity, selectivity and process yield have been studied in detail. The comparison of the results obtained with literature data available for Protein A bead-based chromatography shows that the two processes have similar performance and these membranes can be used to overcome the throughput limitations and other well known drawbacks of traditional bead-based chromatographic columns.

C. Boi, S. Dimartino, G.C. Sarti (2008). Isolamento primario di anticorpi monoclonali con membrane di affinità. s.l : GRICU.

Isolamento primario di anticorpi monoclonali con membrane di affinità

BOI, CRISTIANA;DIMARTINO, SIMONE;SARTI, GIULIO CESARE
2008

Abstract

Affinity membrane chromatography is one of the processes considered as an alternative to conventional Protein A bead-based chromatography for the primary capture step of a monoclonal antibody production process. In this work, new affinity membranes endowed with a very interesting binding capacity for human IgG have been experimentally characterized. The membranes have been extensively tested in dynamic experiments with pure IgG solutions and with a cell culture supernatant containing IgG1. The effects of flow rate and IgG concentration in the feed on the separation performances like binding capacity, selectivity and process yield have been studied in detail. The comparison of the results obtained with literature data available for Protein A bead-based chromatography shows that the two processes have similar performance and these membranes can be used to overcome the throughput limitations and other well known drawbacks of traditional bead-based chromatographic columns.
2008
Atti del convegno Gr.I.C.U. 2008
57
62
C. Boi, S. Dimartino, G.C. Sarti (2008). Isolamento primario di anticorpi monoclonali con membrane di affinità. s.l : GRICU.
C. Boi; S. Dimartino; G.C. Sarti
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/70851
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