Objectives: Stem cells are potential agents for the treatment of myocardial infarction and Bone Marrow Cells (BMC) are the most extensively investigated. Engraftment and delivery strategies are still controversial. We present an in vivo experimental model with a swine animal. Technical aspects as, infarction induction and cell transplantation with a biopolymer scaffold, anesthesia management, diagnosis and preliminary results are described Materials and methods: Infarction is created by direct ligature of the interventricularis artery. Simultaneously, BMC are harvested. BMC are left grown during two weeks and are marked with bromodeoxyirudine. Then are sown in a polymeric scaffold surface of hyaluronic acid (HYAFF-11) and left for 15 days. Thirty days after the infarction, a second operation is performed. Through right thoracotomy, the heart is exposed and an intra-myocardial pocket created at the scar level. The scaffold in then implanted inside, and the myocardial flap fixed above. To evaluate ventricular function and wall kinesis, a Q-contrast echocardiography with CEUS method is performed. The first one is performed the day before the infarction, the second one, 30 days later and the last one, 4 weeks after the biopolymer implantation. The animal is then suppressed and histological analysis performed. Until now, infarction has been performed in 17 swines. The scaffold was implanted in 6 cases, with BMC in 4 cases, and without in other 2 cases. Results: Histology demonstrated, in those cases of biopolymer implantation plus BMC, an increased angiogenesis. Echocardiography with Q-contrast shown an increased perfusion in the infarcted area. Conclusions: This experimental model represents an optimal and reproducible “in vivo” strategy of stem cell transplantation or delivery. Our preliminary results confirm the validity of the animal model, in terms of infarction generation and BMC engraftment. Bigger population as well as further investigation is still needed to understand the ongoing neo-angiogenesis and the myocardial regeneration.
martin suarez s., foschi f., muscari c., capitani o., valgimigli s., fiorelli f., et al. (2009). transplantation of a hyaluronan-based biopolymer engineerized with autologous stem cells in pig ischemic myocardium: an "in vivo" experimental model.. s.l : s.n.
transplantation of a hyaluronan-based biopolymer engineerized with autologous stem cells in pig ischemic myocardium: an "in vivo" experimental model.
MUSCARI, CLAUDIO;CAPITANI, OMBRETTA;VALGIMIGLI, SIMOND;FIORELLI, FEDERICO;ARPESELLA, GIORGIO;PASQUINELLI, GIANANDREA;
2009
Abstract
Objectives: Stem cells are potential agents for the treatment of myocardial infarction and Bone Marrow Cells (BMC) are the most extensively investigated. Engraftment and delivery strategies are still controversial. We present an in vivo experimental model with a swine animal. Technical aspects as, infarction induction and cell transplantation with a biopolymer scaffold, anesthesia management, diagnosis and preliminary results are described Materials and methods: Infarction is created by direct ligature of the interventricularis artery. Simultaneously, BMC are harvested. BMC are left grown during two weeks and are marked with bromodeoxyirudine. Then are sown in a polymeric scaffold surface of hyaluronic acid (HYAFF-11) and left for 15 days. Thirty days after the infarction, a second operation is performed. Through right thoracotomy, the heart is exposed and an intra-myocardial pocket created at the scar level. The scaffold in then implanted inside, and the myocardial flap fixed above. To evaluate ventricular function and wall kinesis, a Q-contrast echocardiography with CEUS method is performed. The first one is performed the day before the infarction, the second one, 30 days later and the last one, 4 weeks after the biopolymer implantation. The animal is then suppressed and histological analysis performed. Until now, infarction has been performed in 17 swines. The scaffold was implanted in 6 cases, with BMC in 4 cases, and without in other 2 cases. Results: Histology demonstrated, in those cases of biopolymer implantation plus BMC, an increased angiogenesis. Echocardiography with Q-contrast shown an increased perfusion in the infarcted area. Conclusions: This experimental model represents an optimal and reproducible “in vivo” strategy of stem cell transplantation or delivery. Our preliminary results confirm the validity of the animal model, in terms of infarction generation and BMC engraftment. Bigger population as well as further investigation is still needed to understand the ongoing neo-angiogenesis and the myocardial regeneration.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
