According to the literature, in hexaploid wheat resistance to CSBMV is controlled by a few major genes. In field trials carried out for many years in Italy, most of the cultivars of hexaploid and durum wheat marketed in this country exhibited a consistent array of intermediate reactions to the virus, suggesting that, in these wheats, resistance to CSBMV is governed by numerous genes. A population consisting of 184 RILs (at the F7 generation) obtained by Produttori Sementi Bologna Spa, Italy, from a cross between the durum wheat cultivars Meridiano (classed as either resistant or moderately resistant to CSBMV in various seasons) and Claudio (repeatedly classed as moderately susceptible) was grown during the 2006–07 season in a field near Cadriano (Bologna) with natural inoculum sources of CSBMV and evaluated for resistance on the basis of both symptomatology (on a 0–4 scale) and virus Concentration (by ELISA). Disease pressure at the experimental site was severe, as testified by the relatively high percentage of lines (6%) with symptom severity scores equal or above 3.5. The results obtained showed that in the ‘Meridiano / Claudio’ cross, resistance to CSBMV is controlled by no less than four genes and that genotypes expressing more extreme reactions than either parent may be recovered (Tables 4 and 5). Our results also strongly suggested the presence of a further gene that, regardless of the symptomatology and virus concentration displayed on the first observation date, has a marked impact on the subsequent reaction to CSBMV of each line, i.e., a plus or minus effect of about 0.250 in terms of ELISA value and of about 0.2 in terms of symptom severity expression. The ‘Meridiano / Claudio’ population will be evaluated again for CSBMV-resistance in the same field during the 2007–08 season. Presently, the population is being profiled with SSR and DArT markers. Analysis of phenotypic and molecular data will allow us to identify the QTL involved in the control of CSBMV resistance.
Inheritance of resistance to cereal soil-borne mosaic virus a durum wheat population of lines derived from the cross ‘Meridiano / Claudio’ / Ratti C., C. Rubies-Autonell, M. Maccaferri, S. Corneti, S. Stefanelli, M.C. Sanguineti; A. Demontis, A. Massi, V. Vallega.. - In: ANNUAL WHEAT NEWSLETTER. - STAMPA. - 55:(2008), pp. 106-108.
Inheritance of resistance to cereal soil-borne mosaic virus a durum wheat population of lines derived from the cross ‘Meridiano / Claudio’
RATTI, CLAUDIO;RUBIES AUTONELL, CONCEPCION;MACCAFERRI, MARCO;STEFANELLI, SANDRA;SANGUINETI, MARIA CORINNA;
2008
Abstract
According to the literature, in hexaploid wheat resistance to CSBMV is controlled by a few major genes. In field trials carried out for many years in Italy, most of the cultivars of hexaploid and durum wheat marketed in this country exhibited a consistent array of intermediate reactions to the virus, suggesting that, in these wheats, resistance to CSBMV is governed by numerous genes. A population consisting of 184 RILs (at the F7 generation) obtained by Produttori Sementi Bologna Spa, Italy, from a cross between the durum wheat cultivars Meridiano (classed as either resistant or moderately resistant to CSBMV in various seasons) and Claudio (repeatedly classed as moderately susceptible) was grown during the 2006–07 season in a field near Cadriano (Bologna) with natural inoculum sources of CSBMV and evaluated for resistance on the basis of both symptomatology (on a 0–4 scale) and virus Concentration (by ELISA). Disease pressure at the experimental site was severe, as testified by the relatively high percentage of lines (6%) with symptom severity scores equal or above 3.5. The results obtained showed that in the ‘Meridiano / Claudio’ cross, resistance to CSBMV is controlled by no less than four genes and that genotypes expressing more extreme reactions than either parent may be recovered (Tables 4 and 5). Our results also strongly suggested the presence of a further gene that, regardless of the symptomatology and virus concentration displayed on the first observation date, has a marked impact on the subsequent reaction to CSBMV of each line, i.e., a plus or minus effect of about 0.250 in terms of ELISA value and of about 0.2 in terms of symptom severity expression. The ‘Meridiano / Claudio’ population will be evaluated again for CSBMV-resistance in the same field during the 2007–08 season. Presently, the population is being profiled with SSR and DArT markers. Analysis of phenotypic and molecular data will allow us to identify the QTL involved in the control of CSBMV resistance.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
