The representativeness of a cellular model is fundamental in pre-clinical cancer studies. Size, heterogeneity and perfusion are three key aspects characterizing the behavior of the tumor and driving its progression. In vitro resemblance of in vivo tumor conditions can be maximized by: (a) using heterogeneous large-sized three-dimensional (3D) multicellular models; (b) utilizing fluidic systems to modulate the culture microenvironment. This work discusses the benefits of using large-sized spheroids as 3D pre-clinical culture models, besides analyzing the microfluidic systems that permit their cultivation and manipulation in dynamic controlled conditions.

Advances in cancer modeling: fluidic systems for increasing representativeness of large 3D multicellular spheroids / Filippo Piccinini, Ilaria De Santis, Alessandro Bevilacqua. - In: BIOTECHNIQUES. - ISSN 0736-6205. - ELETTRONICO. - 65:6(2018), pp. 312-314. [10.2144/btn-2018-0153]

Advances in cancer modeling: fluidic systems for increasing representativeness of large 3D multicellular spheroids

Filippo Piccinini;Ilaria De Santis;Alessandro Bevilacqua
2018

Abstract

The representativeness of a cellular model is fundamental in pre-clinical cancer studies. Size, heterogeneity and perfusion are three key aspects characterizing the behavior of the tumor and driving its progression. In vitro resemblance of in vivo tumor conditions can be maximized by: (a) using heterogeneous large-sized three-dimensional (3D) multicellular models; (b) utilizing fluidic systems to modulate the culture microenvironment. This work discusses the benefits of using large-sized spheroids as 3D pre-clinical culture models, besides analyzing the microfluidic systems that permit their cultivation and manipulation in dynamic controlled conditions.
2018
Advances in cancer modeling: fluidic systems for increasing representativeness of large 3D multicellular spheroids / Filippo Piccinini, Ilaria De Santis, Alessandro Bevilacqua. - In: BIOTECHNIQUES. - ISSN 0736-6205. - ELETTRONICO. - 65:6(2018), pp. 312-314. [10.2144/btn-2018-0153]
Filippo Piccinini, Ilaria De Santis, Alessandro Bevilacqua
File in questo prodotto:
File Dimensione Formato  
btn-2018-0153.pdf

accesso aperto

Tipo: Versione (PDF) editoriale
Licenza: Licenza per Accesso Aperto. Creative Commons Attribuzione - Non commerciale - Non opere derivate (CCBYNCND)
Dimensione 1.33 MB
Formato Adobe PDF
1.33 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/646541
Citazioni
  • ???jsp.display-item.citation.pmc??? 4
  • Scopus 6
  • ???jsp.display-item.citation.isi??? 6
social impact