Background: The contribution of tear protein expression in patients with presumed diagnosis of Sjogren syndrome is underestimated. We aimed to evaluate the role of tear proteins in the Sjogren syndrome early diagnosis. Methods: Charts from 110 patients suspected of Sjogren syndrome were analysed and the subsequent diagnosis retrieved. Subjective symptoms (ocular surface disease index, OSDI), tear film break-up time (TFBUT), Schirmer test, Jones test, tear clearance (TC), corneal (NEI score) and conjunctival staining (van Bjerstelveldt score), esthesiometry, cytology, tear protein analysis (total protein [TP] content, lysozyme-C [LYS-C], lactoferrin [LACTO], lipocalin-I [LIPOC-I] and albumin [ALB]) were analysed. The diagnostic performance with area under the curve (AUC) and odds ratio (OR) for each parameter were calculated. Results: Thirty-five patients (31.8%) had been diagnosed as affected by Sjogren syndrome. Clinical tests showed lower diagnostic performance (OSDI > 44 [AUC 0.57], Schirmer <= 5 mm [0.59], TFBUT <= 3 s [0.72], TC > 1/16 [0.68], Jones <= 4 mm [0.68], corneal staining > 2 [0.51], conjunctival staining > 2 [0.78]) compared with tear proteins (LYS-C <= 1.5 mg/mL [0.79], LACTO <= 20% [0.94], LIPOC-1 <= 10% [0.89], ALB >= 15% [0.79]). LYS-C, LACTO, LIPOC-1 and ALB showed a significant association in predicting Sjogren syndrome vs. not-Sjogren syndrome dry eye (OR, respectively, 4.9, 5.5, 7.2, 6.7). Conclusions: Tear proteins' concentrations showed a significant higher accuracy compared with the traditional ocular clinical tests for reaching Sjogren syndrome's diagnosis. In particular, LACTO and LIPOC- I provided an excellent diagnostic performance and thus could likely be considered promising biomarkers of Sjogren syndrome.

ANNALS EXPRESS: Predictive Role of Tear Protein Expression in The Early Diagnosis of Sjögren's Syndrome

Versura, Piera
Writing – Review & Editing
;
Giannaccare, Giuseppe
Writing – Original Draft Preparation
;
Campos, Emilio
Supervision
2018

Abstract

Background: The contribution of tear protein expression in patients with presumed diagnosis of Sjogren syndrome is underestimated. We aimed to evaluate the role of tear proteins in the Sjogren syndrome early diagnosis. Methods: Charts from 110 patients suspected of Sjogren syndrome were analysed and the subsequent diagnosis retrieved. Subjective symptoms (ocular surface disease index, OSDI), tear film break-up time (TFBUT), Schirmer test, Jones test, tear clearance (TC), corneal (NEI score) and conjunctival staining (van Bjerstelveldt score), esthesiometry, cytology, tear protein analysis (total protein [TP] content, lysozyme-C [LYS-C], lactoferrin [LACTO], lipocalin-I [LIPOC-I] and albumin [ALB]) were analysed. The diagnostic performance with area under the curve (AUC) and odds ratio (OR) for each parameter were calculated. Results: Thirty-five patients (31.8%) had been diagnosed as affected by Sjogren syndrome. Clinical tests showed lower diagnostic performance (OSDI > 44 [AUC 0.57], Schirmer <= 5 mm [0.59], TFBUT <= 3 s [0.72], TC > 1/16 [0.68], Jones <= 4 mm [0.68], corneal staining > 2 [0.51], conjunctival staining > 2 [0.78]) compared with tear proteins (LYS-C <= 1.5 mg/mL [0.79], LACTO <= 20% [0.94], LIPOC-1 <= 10% [0.89], ALB >= 15% [0.79]). LYS-C, LACTO, LIPOC-1 and ALB showed a significant association in predicting Sjogren syndrome vs. not-Sjogren syndrome dry eye (OR, respectively, 4.9, 5.5, 7.2, 6.7). Conclusions: Tear proteins' concentrations showed a significant higher accuracy compared with the traditional ocular clinical tests for reaching Sjogren syndrome's diagnosis. In particular, LACTO and LIPOC- I provided an excellent diagnostic performance and thus could likely be considered promising biomarkers of Sjogren syndrome.
2018
Versura, Piera; Giannaccare, Giuseppe; Vukatana, Gentiana; Mulè, Rita; Malavolta, Nazzarena; Campos, Emilio
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/645387
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