Cocaine is currently one of the most widespread abuse drugs in the world. Since hair cocaine concentrations are a reliable marker of exposition to the drug, an original liquid chromatographic method has been developed for the determination of cocaine in human hair. The chromatographic analysis was carried out on a Hydro-RP C18 column, using a mobile phase containing a phosphate buffer (pH 3.0) - acetonitrile - methanol (75:15:10, v/v/v). Native cocaine fluorescence was monitored at 315 nm while exciting at 230 nm. Mirtazapine was used as the internal standard. Sample pre-treatment was carried out by incubative extraction with 0.1 M HCl followed by solid-phase extraction with C2 cartridges. Good linearity was obtained over a working range of 0.3-100.0 ng/mg. Both extraction yields (> 90%) and precision values (RSD < 6.2%) were highly satisfactory. The method was successfully applied to hair samples collected from cocaine users. Thus, the method is suitable for the long-term monitoring of cocaine use by means of hair testing.

Quantitative analysis of cocaine in human hair by HPLC with fluorescence detection / L. Mercolini; R. Mandrioli; B. Saladini; M. Conti; C. Baccini; M.A. Raggi. - In: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS. - ISSN 0731-7085. - STAMPA. - 48(2):(2008), pp. 456-461. [10.1016/j.jpba.2008.02.007]

Quantitative analysis of cocaine in human hair by HPLC with fluorescence detection

MERCOLINI, LAURA;MANDRIOLI, ROBERTO;SALADINI, BRUNO;RAGGI, MARIA AUGUSTA
2008

Abstract

Cocaine is currently one of the most widespread abuse drugs in the world. Since hair cocaine concentrations are a reliable marker of exposition to the drug, an original liquid chromatographic method has been developed for the determination of cocaine in human hair. The chromatographic analysis was carried out on a Hydro-RP C18 column, using a mobile phase containing a phosphate buffer (pH 3.0) - acetonitrile - methanol (75:15:10, v/v/v). Native cocaine fluorescence was monitored at 315 nm while exciting at 230 nm. Mirtazapine was used as the internal standard. Sample pre-treatment was carried out by incubative extraction with 0.1 M HCl followed by solid-phase extraction with C2 cartridges. Good linearity was obtained over a working range of 0.3-100.0 ng/mg. Both extraction yields (> 90%) and precision values (RSD < 6.2%) were highly satisfactory. The method was successfully applied to hair samples collected from cocaine users. Thus, the method is suitable for the long-term monitoring of cocaine use by means of hair testing.
2008
Quantitative analysis of cocaine in human hair by HPLC with fluorescence detection / L. Mercolini; R. Mandrioli; B. Saladini; M. Conti; C. Baccini; M.A. Raggi. - In: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS. - ISSN 0731-7085. - STAMPA. - 48(2):(2008), pp. 456-461. [10.1016/j.jpba.2008.02.007]
L. Mercolini; R. Mandrioli; B. Saladini; M. Conti; C. Baccini; M.A. Raggi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/64195
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