Nosema ceranae is now a widespread honey bee pathogen with high incidence in apiculture. Rapid and reliable detection and quantification methods are a matter of concern for research community, nowadays mainly relying on the use of biomolecular techniques such as PCR, RT-PCR or HRMA. The aim of this technical paper is to provide a new qPCR assay, based on the highly-conserved protein coding gene Hsp70, to detect and quantify the microsporidian Nosema ceranae affecting the western honey bee Apis mellifera. The validation steps to assess efficiency, sensitivity, specificity and robustness of the assay are described also.
Cilia, G., Cabbri, R., Maiorana, G., Cardaio, I., Dall'Olio, R., Nanetti, A. (2018). A novel TaqMan®assay for Nosema ceranae quantification in honey bee, based on the protein coding gene Hsp70. EUROPEAN JOURNAL OF PROTISTOLOGY, 63, 44-50 [10.1016/j.ejop.2018.01.007].
A novel TaqMan®assay for Nosema ceranae quantification in honey bee, based on the protein coding gene Hsp70
Cabbri, Riccardo;DALL'OLIO, RAFFAELE;
2018
Abstract
Nosema ceranae is now a widespread honey bee pathogen with high incidence in apiculture. Rapid and reliable detection and quantification methods are a matter of concern for research community, nowadays mainly relying on the use of biomolecular techniques such as PCR, RT-PCR or HRMA. The aim of this technical paper is to provide a new qPCR assay, based on the highly-conserved protein coding gene Hsp70, to detect and quantify the microsporidian Nosema ceranae affecting the western honey bee Apis mellifera. The validation steps to assess efficiency, sensitivity, specificity and robustness of the assay are described also.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
