The present paper describes the development of an integrated lab-on-chip, in which viral DNA amplification with real-time on-chip detection is carried out under constant temperature of 65°C. The lab-on-chip is composed of a disposable 10-μL polydimethylsiloxane reaction chamber which is thermally and optically coupled to a glass substrate that hosts a thin-film metallic resistive heater and thin-film amorphous silicon diodes which act as temperature and radiation sensors. A loop-mediated isothermal amplification (LAMP) technique was optimized to specifically amplify parvovirus B19 DNA and coupled with Bioluminescent Assay in Real Time (BART) technology to provide real-time detection of target DNA. The experimental results demonstrate the ability of the proposed device to discriminate among different concentrations of viral DNA with an excellent agreement with standard off-chip methods.

On-chip LAMP-BART reaction for viral DNA real-time bioluminescence detection / Mirasoli, M.; Bonvicini, F.; Lovecchio, N.; Petrucci, G.; Zangheri, M.; Calabria, D.; Costantini, F.; Roda, A.; Gallinella, G.; Caputo, D.*; de Cesare, G.; Nascetti, A.. - In: SENSORS AND ACTUATORS. B, CHEMICAL. - ISSN 0925-4005. - STAMPA. - 262:(2018), pp. 1024-1033. [10.1016/j.snb.2018.02.086]

On-chip LAMP-BART reaction for viral DNA real-time bioluminescence detection

Mirasoli, M.;Bonvicini, F.;Zangheri, M.;Calabria, D.;Roda, A.;Gallinella, G.;
2018

Abstract

The present paper describes the development of an integrated lab-on-chip, in which viral DNA amplification with real-time on-chip detection is carried out under constant temperature of 65°C. The lab-on-chip is composed of a disposable 10-μL polydimethylsiloxane reaction chamber which is thermally and optically coupled to a glass substrate that hosts a thin-film metallic resistive heater and thin-film amorphous silicon diodes which act as temperature and radiation sensors. A loop-mediated isothermal amplification (LAMP) technique was optimized to specifically amplify parvovirus B19 DNA and coupled with Bioluminescent Assay in Real Time (BART) technology to provide real-time detection of target DNA. The experimental results demonstrate the ability of the proposed device to discriminate among different concentrations of viral DNA with an excellent agreement with standard off-chip methods.
2018
On-chip LAMP-BART reaction for viral DNA real-time bioluminescence detection / Mirasoli, M.; Bonvicini, F.; Lovecchio, N.; Petrucci, G.; Zangheri, M.; Calabria, D.; Costantini, F.; Roda, A.; Gallinella, G.; Caputo, D.*; de Cesare, G.; Nascetti, A.. - In: SENSORS AND ACTUATORS. B, CHEMICAL. - ISSN 0925-4005. - STAMPA. - 262:(2018), pp. 1024-1033. [10.1016/j.snb.2018.02.086]
Mirasoli, M.; Bonvicini, F.; Lovecchio, N.; Petrucci, G.; Zangheri, M.; Calabria, D.; Costantini, F.; Roda, A.; Gallinella, G.; Caputo, D.*; de Cesare, G.; Nascetti, A.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/631330
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