Furoin, a benzoin analogue, was examined as novel fluorogenic reagent for the selective and sensitive LC determination of various guanidines after pre-column derivatization. The derivatization reaction was carried out at 100°C for 5 min to give adducts that were separated on a Phenomenex Synergi MAX-RP column. The reagent showed to be useful both for determining together arginine (Arg) and creatine (CT) in dietary supplements under elution isocratic conditions and for the simultaneous analysis of a variety of guanidines in biological samples (human plasma and urine) under elution gradient conditions. The detection limits ranged from 7 to 25 fmol. Recovery studies showed good results for all determined guanidino compounds (85.6%-106.2%; R.S.D.= 1.1-6.2%).
R.Gatti*, M.G.Gioia (2008). Liquid chromatographic analysis of guanidino compounds using furoin as a new fluorogenic reagent. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 48, 754-759 [10.1016/j.jpba.2008.07.017].
Liquid chromatographic analysis of guanidino compounds using furoin as a new fluorogenic reagent
GATTI, RITA;GIOIA, MARIA GRAZIA
2008
Abstract
Furoin, a benzoin analogue, was examined as novel fluorogenic reagent for the selective and sensitive LC determination of various guanidines after pre-column derivatization. The derivatization reaction was carried out at 100°C for 5 min to give adducts that were separated on a Phenomenex Synergi MAX-RP column. The reagent showed to be useful both for determining together arginine (Arg) and creatine (CT) in dietary supplements under elution isocratic conditions and for the simultaneous analysis of a variety of guanidines in biological samples (human plasma and urine) under elution gradient conditions. The detection limits ranged from 7 to 25 fmol. Recovery studies showed good results for all determined guanidino compounds (85.6%-106.2%; R.S.D.= 1.1-6.2%).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
