PRKAG3 is a major gene for meat quality and production in pigs. In this study, we investigated two single nucleotide polymorphisms (SNPs) of the PRKAG3 promoter region (g.-995A>G and g.-311A>G) in 381 pigs of different breeds. Association between these SNPs and three major haplotypes, constructed including other three substitutions (c.89C>A, c.154A>G and c.595G>A) and different traits (meat quality parameters: pH2h, pHu, glycogen and lactate content, glycolytic potential and cathepsin B activity of Semimembranosus muscle; estimated breeding values and random residuals for growth performances and carcass traits) were analysed in 271 Italian Large White performance tested pigs. A significant effect (P = 0.043) of the g.-995A>G SNP on cathepsin B activity and a suggestive effect (P = 0.067) of the g.-311A>G SNP on pHuwere found. The haplotype [g.-995G:g.-311G:c.89A:c.154G:c.595G] was associated with pHu(P = 0.024), glycolytic potential (P = 0.040) and cathepsin B activity (P = 0.086). This study on haplotypes, including promoter region SNPs, contribute to explain the effect of this gene on meat quality traits in pigs.

Effects of single nucleotide polymorphisms and haplotypes of the protein kinase AMP-activated non-catalytic subunit gamma 3 (PRKAG3) gene on production, meat quality and carcass traits in Italian Large White pigs

Dall'Olio, Stefania
;
Scotti, Emilio;Costa, Leonardo Nanni;Fontanesi, Luca
2018

Abstract

PRKAG3 is a major gene for meat quality and production in pigs. In this study, we investigated two single nucleotide polymorphisms (SNPs) of the PRKAG3 promoter region (g.-995A>G and g.-311A>G) in 381 pigs of different breeds. Association between these SNPs and three major haplotypes, constructed including other three substitutions (c.89C>A, c.154A>G and c.595G>A) and different traits (meat quality parameters: pH2h, pHu, glycogen and lactate content, glycolytic potential and cathepsin B activity of Semimembranosus muscle; estimated breeding values and random residuals for growth performances and carcass traits) were analysed in 271 Italian Large White performance tested pigs. A significant effect (P = 0.043) of the g.-995A>G SNP on cathepsin B activity and a suggestive effect (P = 0.067) of the g.-311A>G SNP on pHuwere found. The haplotype [g.-995G:g.-311G:c.89A:c.154G:c.595G] was associated with pHu(P = 0.024), glycolytic potential (P = 0.040) and cathepsin B activity (P = 0.086). This study on haplotypes, including promoter region SNPs, contribute to explain the effect of this gene on meat quality traits in pigs.
Dall'Olio, Stefania*; Scotti, Emilio; Costa, Leonardo Nanni; Fontanesi, Luca
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/631130
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