The strictly maternal inheritance (SMI) is a pattern of mitochondrial inheritance observed across the whole animal kingdom. However, some interesting exceptions are known for the class Bivalvia, in which several species show an unusual pattern called doubly uniparental inheritance (DUI) whose outcome is a heteroplasmic pool of mtDNA in males. Even if DUI has been studied for long, its molecular basis has not been established yet. The aim of this work is to select classes of proteins known to be involved in the maintenance of SMI and to compare their features in two clam species differing for their mitochondrial inheritance mechanism, that is, the SMI species Ruditapes decussatus and the DUI species Ruditapes philippinarum. Data have been obtained from the transcriptomes of male and female ripe gonads of both species. Our analysis focused on nucleases and polymerases, ubiquitination and ubiquitin-like modifier pathways, and proteins involved in autophagy and mitophagy. For each protein group of interest, transcription bias (male or female), annotation, and mitochondrial targeting (when appropriate) were assessed. We did not find evidence supporting a role of nucleases/polymerases or autophagic machinery in the enforcement of SMI in R. decussatus. On the other hand, ubiquitinating enzymes with the expected features have been retrieved, providing us with two alternative testable models for mitochondrial inheritance mechanisms at the molecular level.

Lose it or keep it: (how bivalves can provide) insights into mitochondrial inheritance mechanisms

PUNZI, ELISABETTA;Milani, Liliana;Ghiselli, Fabrizio;Passamonti, Marco
2018

Abstract

The strictly maternal inheritance (SMI) is a pattern of mitochondrial inheritance observed across the whole animal kingdom. However, some interesting exceptions are known for the class Bivalvia, in which several species show an unusual pattern called doubly uniparental inheritance (DUI) whose outcome is a heteroplasmic pool of mtDNA in males. Even if DUI has been studied for long, its molecular basis has not been established yet. The aim of this work is to select classes of proteins known to be involved in the maintenance of SMI and to compare their features in two clam species differing for their mitochondrial inheritance mechanism, that is, the SMI species Ruditapes decussatus and the DUI species Ruditapes philippinarum. Data have been obtained from the transcriptomes of male and female ripe gonads of both species. Our analysis focused on nucleases and polymerases, ubiquitination and ubiquitin-like modifier pathways, and proteins involved in autophagy and mitophagy. For each protein group of interest, transcription bias (male or female), annotation, and mitochondrial targeting (when appropriate) were assessed. We did not find evidence supporting a role of nucleases/polymerases or autophagic machinery in the enforcement of SMI in R. decussatus. On the other hand, ubiquitinating enzymes with the expected features have been retrieved, providing us with two alternative testable models for mitochondrial inheritance mechanisms at the molecular level.
JOURNAL OF EXPERIMENTAL ZOOLOGY. PART B, MOLECULAR AND DEVELOPMENTAL EVOLUTION
Punzi, Elisabetta; Milani, Liliana; Ghiselli, Fabrizio*; Passamonti, Marco
File in questo prodotto:
File Dimensione Formato  
Punzi-et-al_2017.pdf

embargo fino al 02/02/2019

Tipo: Postprint
Licenza: Licenza per accesso libero gratuito
Dimensione 1.25 MB
Formato Adobe PDF
1.25 MB Adobe PDF Visualizza/Apri
Punzi_et_al_JEZB_2017_SuppMat1.doc

accesso aperto

Tipo: File Supplementare
Licenza: Licenza per Accesso Aperto. Creative Commons Attribuzione - Non commerciale (CCBYNC)
Dimensione 38 kB
Formato Microsoft Word
38 kB Microsoft Word Visualizza/Apri
Punzi_et_al_JEZB_2017_TableS1.xlsx

accesso aperto

Tipo: File Supplementare
Licenza: Licenza per Accesso Aperto. Creative Commons Attribuzione - Non commerciale (CCBYNC)
Dimensione 524.39 kB
Formato Microsoft Excel XML
524.39 kB Microsoft Excel XML Visualizza/Apri
Punzi_et_al_JEZB_2017_TableS2.xlsx

accesso aperto

Tipo: File Supplementare
Licenza: Licenza per Accesso Aperto. Creative Commons Attribuzione - Non commerciale (CCBYNC)
Dimensione 504.28 kB
Formato Microsoft Excel XML
504.28 kB Microsoft Excel XML Visualizza/Apri
Punzi_et_al_JEZB_2017_SuppMatS4.xlsx

accesso aperto

Tipo: File Supplementare
Licenza: Licenza per Accesso Aperto. Creative Commons Attribuzione - Non commerciale (CCBYNC)
Dimensione 9.7 kB
Formato Microsoft Excel XML
9.7 kB Microsoft Excel XML Visualizza/Apri
Punzi_et_al_JEZB_2017_SuppMatS5.xlsx

accesso aperto

Tipo: File Supplementare
Licenza: Licenza per Accesso Aperto. Creative Commons Attribuzione - Non commerciale (CCBYNC)
Dimensione 11.75 kB
Formato Microsoft Excel XML
11.75 kB Microsoft Excel XML Visualizza/Apri
Punzi_et_al_JEZB_2017_SuppMatS6.xlsx

accesso aperto

Tipo: File Supplementare
Licenza: Licenza per Accesso Aperto. Creative Commons Attribuzione - Non commerciale (CCBYNC)
Dimensione 19.02 kB
Formato Microsoft Excel XML
19.02 kB Microsoft Excel XML Visualizza/Apri
Punzi_et_al_JEZB_2017_SuppMatS7.xlsx

accesso aperto

Tipo: File Supplementare
Licenza: Licenza per Accesso Aperto. Creative Commons Attribuzione - Non commerciale (CCBYNC)
Dimensione 18.67 kB
Formato Microsoft Excel XML
18.67 kB Microsoft Excel XML Visualizza/Apri
Punzi_et_al_JEZB_2017_SuppMatS8.xlsx

accesso aperto

Tipo: File Supplementare
Licenza: Licenza per Accesso Aperto. Creative Commons Attribuzione - Non commerciale (CCBYNC)
Dimensione 16.11 kB
Formato Microsoft Excel XML
16.11 kB Microsoft Excel XML Visualizza/Apri
Punzi_et_al_JEZB_2017_SuppMatS9.xlsx

accesso aperto

Tipo: File Supplementare
Licenza: Licenza per Accesso Aperto. Creative Commons Attribuzione - Non commerciale (CCBYNC)
Dimensione 12.93 kB
Formato Microsoft Excel XML
12.93 kB Microsoft Excel XML Visualizza/Apri
Punzi_et_al_JEZB_2017_SuppMatS10.xlsx

accesso aperto

Tipo: File Supplementare
Licenza: Licenza per Accesso Aperto. Creative Commons Attribuzione - Non commerciale (CCBYNC)
Dimensione 12.09 kB
Formato Microsoft Excel XML
12.09 kB Microsoft Excel XML Visualizza/Apri
Punzi_et_al_JEZB_2017_SuppMatS11.xlsx

accesso aperto

Tipo: File Supplementare
Licenza: Licenza per Accesso Aperto. Creative Commons Attribuzione - Non commerciale (CCBYNC)
Dimensione 63.34 kB
Formato Microsoft Excel XML
63.34 kB Microsoft Excel XML Visualizza/Apri
Punzi_et_al_JEZB_2017_SuppMatS12.xlsx

accesso aperto

Tipo: File Supplementare
Licenza: Licenza per Accesso Aperto. Creative Commons Attribuzione - Non commerciale (CCBYNC)
Dimensione 59.64 kB
Formato Microsoft Excel XML
59.64 kB Microsoft Excel XML Visualizza/Apri
Punzi_et_al_JEZB_2017_SuppMatS13.xlsx

accesso aperto

Tipo: File Supplementare
Licenza: Licenza per Accesso Aperto. Creative Commons Attribuzione - Non commerciale (CCBYNC)
Dimensione 72.86 kB
Formato Microsoft Excel XML
72.86 kB Microsoft Excel XML Visualizza/Apri
Punzi_et_al_JEZB_2017_SuppMatS14.xlsx

accesso aperto

Tipo: File Supplementare
Licenza: Licenza per Accesso Aperto. Creative Commons Attribuzione - Non commerciale (CCBYNC)
Dimensione 66.14 kB
Formato Microsoft Excel XML
66.14 kB Microsoft Excel XML Visualizza/Apri
Punzi_et_al_JEZB_2017_SuppMatS15.xlsx

accesso aperto

Tipo: File Supplementare
Licenza: Licenza per Accesso Aperto. Creative Commons Attribuzione - Non commerciale (CCBYNC)
Dimensione 167.83 kB
Formato Microsoft Excel XML
167.83 kB Microsoft Excel XML Visualizza/Apri
Punzi_et_al_JEZB_2017_SuppMatS16.xlsx

accesso aperto

Tipo: File Supplementare
Licenza: Licenza per Accesso Aperto. Creative Commons Attribuzione - Non commerciale (CCBYNC)
Dimensione 158.45 kB
Formato Microsoft Excel XML
158.45 kB Microsoft Excel XML Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11585/629013
Citazioni
  • ???jsp.display-item.citation.pmc??? 2
  • Scopus 8
  • ???jsp.display-item.citation.isi??? 8
social impact