The relationship among sperm attributes of DNA integrity, sperm motility, morphology,viability, acrosome integrity and in vivo fertility of frozen-thawed Italian MediterraneanBuffalo (IMB) sperm has not been reported. Straws of frozen-thawed semen samplesfrom three bulls were examined. Sperm DNA assays (i.e., neutral Comet assay, SpermBos Halomax-SBH and Sperm Chromatin Structure Assay-SCSA) were not correlated toeach other (P > 0.05). Many neutral Comet assay measures were correlated to total spermmotility-TMOT (% head-H-DNA, r = 0.74; Olive moment, r = −0.76; P < 0.05) and coiled tails(r-values ranged from% H-DNA, r = −0.80 to tail length, r = −0.71; P < 0.05). The COMP-twasnegatively correlated to viable acrosome intact (VAI) sperm, and distal droplets (r = −0.60and −0.61; P < 0.05), whereas Mean-tand Mode-twere positively correlated to bent mid-pieces (r = 0.63 and 0.61; P < 0.05). The SBH assay was positively correlated to non-viableacrosome damaged (NVAD) sperm (r = 0.60; P < 0.05) and negatively correlated to viableacrosome damaged (VAD) sperm (r = −0.63; P < 0.05). The overall pregnancy rate (PR-at 30and 45 d post artificial insemination-AI) and the calving rate were 57%, 55% and 45%, respec-tively. Among sperm features analyzed the area under the Receiver Operating Characteristic(ROC) Curve was significant (P < 0.05) for TMOT, NVAD, Standard Deviation-t(SD-t) andneutral comet measures (Olive tail moment and tail moment, % H- DNA and tail area) inestimating pregnancy.

Sperm DNA integrity in frozen-thawed semen from italian mediterranean buffalo bulls and its relationship to in vivo fertility

SERAFINI, MARIA ROSSELLA
;
Mari G.;Mislei B.;LO CASO, CHIARA;
2016

Abstract

The relationship among sperm attributes of DNA integrity, sperm motility, morphology,viability, acrosome integrity and in vivo fertility of frozen-thawed Italian MediterraneanBuffalo (IMB) sperm has not been reported. Straws of frozen-thawed semen samplesfrom three bulls were examined. Sperm DNA assays (i.e., neutral Comet assay, SpermBos Halomax-SBH and Sperm Chromatin Structure Assay-SCSA) were not correlated toeach other (P > 0.05). Many neutral Comet assay measures were correlated to total spermmotility-TMOT (% head-H-DNA, r = 0.74; Olive moment, r = −0.76; P < 0.05) and coiled tails(r-values ranged from% H-DNA, r = −0.80 to tail length, r = −0.71; P < 0.05). The COMP-twasnegatively correlated to viable acrosome intact (VAI) sperm, and distal droplets (r = −0.60and −0.61; P < 0.05), whereas Mean-tand Mode-twere positively correlated to bent mid-pieces (r = 0.63 and 0.61; P < 0.05). The SBH assay was positively correlated to non-viableacrosome damaged (NVAD) sperm (r = 0.60; P < 0.05) and negatively correlated to viableacrosome damaged (VAD) sperm (r = −0.63; P < 0.05). The overall pregnancy rate (PR-at 30and 45 d post artificial insemination-AI) and the calving rate were 57%, 55% and 45%, respec-tively. Among sperm features analyzed the area under the Receiver Operating Characteristic(ROC) Curve was significant (P < 0.05) for TMOT, NVAD, Standard Deviation-t(SD-t) andneutral comet measures (Olive tail moment and tail moment, % H- DNA and tail area) inestimating pregnancy.
2016
Serafini R., Love CC., Coletta A., Mari G., Mislei B., Caso C., Di Palo R
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/628120
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