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EnglishThe suitability of competitive enzyme-linked immunosorbent assays (ELISAs) with chemiluminescent detection based immobilized antigen (indirect assay) for rapid an accurate determination of chlorpyrifos in different food matrices was tested. The limit of detection (LOD) value were between 1-1.75 ng ml-1, the standard curve midpoint (IC50) was 3.5 ng ml-1, and assay duration was 1.5 h. Assay application to the analysis of honeybees extract resulted in chlorpyrifos recoveries varying between 62 and 83 % in 5-15 ng ml-1 herbicide concentration range.
| Titolo: | Analysis of Chlorpyrifos in Water, Fruit juices and Honeybee Extracts by Chemiluminescent ELISA |
| Autore/i: | C. Soler; GIROTTI, STEFANO; GHINI, SEVERINO; FINI, FABIANA; A. Montoya; J. J. Manclús; J. Mañes |
| Autore/i Unibo: | |
| Anno: | 2008 |
| Rivista: | |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1080/00032710802363115 |
| Abstract: | The suitability of competitive enzyme-linked immunosorbent assays (ELISAs) with chemiluminescent detection based immobilized antigen (indirect assay) for rapid an accurate determination of chlorpyrifos in different food matrices was tested. The limit of detection (LOD) value were between 1-1.75 ng ml-1, the standard curve midpoint (IC50) was 3.5 ng ml-1, and assay duration was 1.5 h. Assay application to the analysis of honeybees extract resulted in chlorpyrifos recoveries varying between 62 and 83 % in 5-15 ng ml-1 herbicide concentration range. |
| Data prodotto definitivo in UGOV: | 2009-01-14 18:27:11 |
| Appare nelle tipologie: | 1.01 Articolo in rivista |
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http://hdl.handle.net/11585/62653
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