The suitability of competitive enzyme-linked immunosorbent assays (ELISAs) with chemiluminescent detection based immobilized antigen (indirect assay) for rapid an accurate determination of chlorpyrifos in different food matrices was tested. The limit of detection (LOD) value were between 1-1.75 ng ml-1, the standard curve midpoint (IC50) was 3.5 ng ml-1, and assay duration was 1.5 h. Assay application to the analysis of honeybees extract resulted in chlorpyrifos recoveries varying between 62 and 83 % in 5-15 ng ml-1 herbicide concentration range.
Analysis of Chlorpyrifos in Water, Fruit juices and Honeybee Extracts by Chemiluminescent ELISA
GIROTTI, STEFANO;GHINI, SEVERINO;FINI, FABIANA;
2008
Abstract
The suitability of competitive enzyme-linked immunosorbent assays (ELISAs) with chemiluminescent detection based immobilized antigen (indirect assay) for rapid an accurate determination of chlorpyrifos in different food matrices was tested. The limit of detection (LOD) value were between 1-1.75 ng ml-1, the standard curve midpoint (IC50) was 3.5 ng ml-1, and assay duration was 1.5 h. Assay application to the analysis of honeybees extract resulted in chlorpyrifos recoveries varying between 62 and 83 % in 5-15 ng ml-1 herbicide concentration range.File in questo prodotto:
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