Transglutaminase (TGase:E.C. 2.3.2.13) catalyzes the acyl-transfer reaction between one or two primary amino groups of polyamines and protein-bound Gln residues giving rise to post-translational modifications. One increasing the positive charge on a proteins surface and the other results in the covalent crosslinking of proteins. Pioneering studies on TGase in plants started in the middle of the 1980’s but the methodology designed for use with animal extracts was not directly applicable to plant extracts. Here we describe radioactive and colorimetric methods adapted to study plant TGase, as well as protocols to analyze the involvement of TGase and polyamines in the functionality of cytoskeletal proteins.

Del Duca, S., Bonner, P., Aloisi, I., Serafini-Fracassini, D., Cai, G. (2018). Determination of transglutaminase activity in plants. New York : Humana Press Inc. [10.1007/978-1-4939-7398-9_18].

Determination of transglutaminase activity in plants

Del Duca, S.;Aloisi, I.;Serafini-Fracassini, D.;
2018

Abstract

Transglutaminase (TGase:E.C. 2.3.2.13) catalyzes the acyl-transfer reaction between one or two primary amino groups of polyamines and protein-bound Gln residues giving rise to post-translational modifications. One increasing the positive charge on a proteins surface and the other results in the covalent crosslinking of proteins. Pioneering studies on TGase in plants started in the middle of the 1980’s but the methodology designed for use with animal extracts was not directly applicable to plant extracts. Here we describe radioactive and colorimetric methods adapted to study plant TGase, as well as protocols to analyze the involvement of TGase and polyamines in the functionality of cytoskeletal proteins.
2018
Methods in Molecular Biology
173
200
Del Duca, S., Bonner, P., Aloisi, I., Serafini-Fracassini, D., Cai, G. (2018). Determination of transglutaminase activity in plants. New York : Humana Press Inc. [10.1007/978-1-4939-7398-9_18].
Del Duca, S.*; Bonner, P.L.R.; Aloisi, I.; Serafini-Fracassini, D.; Cai, G.
File in questo prodotto:
Eventuali allegati, non sono esposti

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/625901
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 2
  • Scopus 5
  • ???jsp.display-item.citation.isi??? 5
social impact