β-Galactosidase (β-Gal) activity has been the most extensively utilized biomarker for the detection of cellular senescence. It can be measured also in plasma, and few recent evidence showed an altered plasmatic β-Gal activity in patients affected by some age-related diseases (ARDs). Since T2DM is one of the most common ARDs, we aimed to investigate if plasmatic β-Gal activity is modulated in T2DM patients and if "age" could affect such modulation. To gain mechanistic insights we paralleled this investigation with the evaluation of β-Gal activity in young and senescent endothelial cells (HUVECs) cultured in normo- and hyper-glycaemic environment. A significant age-related increase of plasmatic β-Gal activity was observed in healthy subjects (n. 230; 55-87 years), whereas the enzymatic activity was significantly reduced in T2DM patients (n. 230; 55-96 years) compared to healthy subjects. β-Gal activity detectable both in cells and in the culture medium was significantly increased in senescent cells compared to the younger ones, both under normo- and hyper-glycaemic condition. However, the hyper-glycaemic condition was not associated with an increased β-Gal activity in milieu compared to normo-glycaemic condition. Overall our data reinforce the notion that plasmatic β-Gal activity could be a systemic biomarker of aging, whereas T2DM patients are characterized by a different age-releated trend.

Spazzafumo, L., Mensà, E., Matacchione, G., Galeazzi, T., Zampini, L., Recchioni, R., et al. (2017). Age-related modulation of plasmatic beta-Galactosidase activity in healthy subjects and in patients affected by T2DM. ONCOTARGET, 8(55), 93338-93348 [10.18632/oncotarget.21848].

Age-related modulation of plasmatic beta-Galactosidase activity in healthy subjects and in patients affected by T2DM

Garagnani, Paolo;Bonafè, Massimiliano;Procopio, Antonio Domenico;Olivieri, Fabiola
2017

Abstract

β-Galactosidase (β-Gal) activity has been the most extensively utilized biomarker for the detection of cellular senescence. It can be measured also in plasma, and few recent evidence showed an altered plasmatic β-Gal activity in patients affected by some age-related diseases (ARDs). Since T2DM is one of the most common ARDs, we aimed to investigate if plasmatic β-Gal activity is modulated in T2DM patients and if "age" could affect such modulation. To gain mechanistic insights we paralleled this investigation with the evaluation of β-Gal activity in young and senescent endothelial cells (HUVECs) cultured in normo- and hyper-glycaemic environment. A significant age-related increase of plasmatic β-Gal activity was observed in healthy subjects (n. 230; 55-87 years), whereas the enzymatic activity was significantly reduced in T2DM patients (n. 230; 55-96 years) compared to healthy subjects. β-Gal activity detectable both in cells and in the culture medium was significantly increased in senescent cells compared to the younger ones, both under normo- and hyper-glycaemic condition. However, the hyper-glycaemic condition was not associated with an increased β-Gal activity in milieu compared to normo-glycaemic condition. Overall our data reinforce the notion that plasmatic β-Gal activity could be a systemic biomarker of aging, whereas T2DM patients are characterized by a different age-releated trend.
2017
Spazzafumo, L., Mensà, E., Matacchione, G., Galeazzi, T., Zampini, L., Recchioni, R., et al. (2017). Age-related modulation of plasmatic beta-Galactosidase activity in healthy subjects and in patients affected by T2DM. ONCOTARGET, 8(55), 93338-93348 [10.18632/oncotarget.21848].
Spazzafumo, Liana; Mensà, Emanuela; Matacchione, Giulia; Galeazzi, Tiziana; Zampini, Lucia; Recchioni, Rina; Marcheselli, Fiorella; Prattichizzo, Fran...espandi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/624309
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