The objective of the present work was to characterize changes in calpain activity in pork post-mortem. Samples from pig M. longissimus dorsi and M. semimembranosus were collected three days post-mortem from 75 animals and analyzed with casein zymography. The results indicated post-mortem autolysis of m-calpain as two m-calpain bands were observed on the zymogram gel. Use of M. longissimus dorsi from three pigs collected at different times during storage further confirmed post-mortem autolysis of m-calpain. The activity of the autolyzed form of m-calpain was detectable at day 3 and further increased at day 6. The results also showed a decrease in the non-autolyzed m-calpain activity during post-mortem storage. Collectively, these results suggest that m-calpain is active post-mortem in porcine muscles.

Pomponio L., Lametsch R. , Karlsson A.H., Nanni Costa L., Grossi A., Ertbjerg P. (2008). Evidence for post-mortem m-calpain autolysis in porcine muscle. MEAT SCIENCE, 80, 761-764 [10.1016/j.meatsci.2008.03.019].

Evidence for post-mortem m-calpain autolysis in porcine muscle

NANNI COSTA, LEONARDO;
2008

Abstract

The objective of the present work was to characterize changes in calpain activity in pork post-mortem. Samples from pig M. longissimus dorsi and M. semimembranosus were collected three days post-mortem from 75 animals and analyzed with casein zymography. The results indicated post-mortem autolysis of m-calpain as two m-calpain bands were observed on the zymogram gel. Use of M. longissimus dorsi from three pigs collected at different times during storage further confirmed post-mortem autolysis of m-calpain. The activity of the autolyzed form of m-calpain was detectable at day 3 and further increased at day 6. The results also showed a decrease in the non-autolyzed m-calpain activity during post-mortem storage. Collectively, these results suggest that m-calpain is active post-mortem in porcine muscles.
2008
Pomponio L., Lametsch R. , Karlsson A.H., Nanni Costa L., Grossi A., Ertbjerg P. (2008). Evidence for post-mortem m-calpain autolysis in porcine muscle. MEAT SCIENCE, 80, 761-764 [10.1016/j.meatsci.2008.03.019].
Pomponio L.; Lametsch R. ; Karlsson A.H.; Nanni Costa L.; Grossi A.; Ertbjerg P.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/62394
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