Dielectrophoresis (DEP) is a very valuable approach for designing and developing laboratory-on-a-chip (lab-on-a-chip) devices that are able to manipulate microparticles and cells. Lab-on-a-chip technology will enable laboratory testing to move from laboratories using complex equipment to nonlaboratory settings. We used a lab-on-a-chip device, the SmartSlide, which carries 193 parallel electrodes and generates up to 50 cylinder-shaped DEP cages able to entrap microparticles and cells within DEP cages and move them along the chip. For lab-on-a-chip technology, the characterization of microparticles exhibiting a differential ability to be DEP-caged, levitated, and moved is important for the development of both diagnostic and therapeutic protocols. We determined whether the SmartSlide could be used to levitate and move tripalmitin-based lipospheres carrying increasing concentrations of dihexadecyl dimethyl ammonium bromide (DHDAB) as a cationic surfactant. The data obtained with this DEP-based platform showed that DEP caging, levitation, and movement of the cationic lipospheres depended on the percentage of DHDAB. Tripalmitin lipospheres containing 6% DHDAB could be DEP-caged and manipulated. On the contrary, lipospheres containing 12% DHDAB did not exhibit an efficient ability to be DEP-caged and moved throughout the chip. To our knowledge, this is the first report on the possible use of a DEP-based lab-on-a-chip device for guided manipulation of lipospheres. This information might be of interest in the fields of drug discovery, delivery, and diagnosis. © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2008
Levitation and movement of tripalmitin-based cationic lipospheres on a dielectrophoresis-based lab-on-a-chip device / E.Fabbri; M. Borgatti; N. Manaresi; G. Medoro; C. Nastruzzi; S. Di Croce; A. Tosi; S. Mazzitelli; I. Mancini; R. Guerrieri; R. Gambari. - In: JOURNAL OF APPLIED POLYMER SCIENCE. - ISSN 0021-8995. - ELETTRONICO. - 109 Issue 6:(2008), pp. 3484-3491. [10.1002/app.28413]
Levitation and movement of tripalmitin-based cationic lipospheres on a dielectrophoresis-based lab-on-a-chip device
MANARESI, NICOLO';MEDORO, GIANNI;GUERRIERI, ROBERTO;
2008
Abstract
Dielectrophoresis (DEP) is a very valuable approach for designing and developing laboratory-on-a-chip (lab-on-a-chip) devices that are able to manipulate microparticles and cells. Lab-on-a-chip technology will enable laboratory testing to move from laboratories using complex equipment to nonlaboratory settings. We used a lab-on-a-chip device, the SmartSlide, which carries 193 parallel electrodes and generates up to 50 cylinder-shaped DEP cages able to entrap microparticles and cells within DEP cages and move them along the chip. For lab-on-a-chip technology, the characterization of microparticles exhibiting a differential ability to be DEP-caged, levitated, and moved is important for the development of both diagnostic and therapeutic protocols. We determined whether the SmartSlide could be used to levitate and move tripalmitin-based lipospheres carrying increasing concentrations of dihexadecyl dimethyl ammonium bromide (DHDAB) as a cationic surfactant. The data obtained with this DEP-based platform showed that DEP caging, levitation, and movement of the cationic lipospheres depended on the percentage of DHDAB. Tripalmitin lipospheres containing 6% DHDAB could be DEP-caged and manipulated. On the contrary, lipospheres containing 12% DHDAB did not exhibit an efficient ability to be DEP-caged and moved throughout the chip. To our knowledge, this is the first report on the possible use of a DEP-based lab-on-a-chip device for guided manipulation of lipospheres. This information might be of interest in the fields of drug discovery, delivery, and diagnosis. © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2008I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.