A structurally correlated series of cell-permeant thiophene fluorophores, characterized by intense green or red fluorescence inside live mouse embryonic fibroblasts, was developed. The fluorophores displayed rapid internalization, excellent retention inside the cells, and high optical stability in the cytosolic environment and did not alter cell viability and reproducibility. Depending on the molecular structure, they experienced distinct fate inside the cells: from bright and lasting staining of the cytoplasm to selective tagging of a small set of globular proteins. © The Royal Society of Chemistry 2014.

Live cell cytoplasm staining and selective labeling of intracellular proteins by non-toxic cell-permeant thiophene fluorophores / DI MARIA, FRANCESCA GIULIA; Palamã , I. E.; Baroncini, Massimo; Barbieri, A.; Bongini, Alessandro; Bizzarri, R.; Gigli, G.; Barbarella, G.. - In: ORGANIC & BIOMOLECULAR CHEMISTRY. - ISSN 1477-0520. - STAMPA. - 12:10(2014), pp. 1603-1610. [10.1039/c3ob41982g]

Live cell cytoplasm staining and selective labeling of intracellular proteins by non-toxic cell-permeant thiophene fluorophores

Di Maria, F.;Baroncini, M.;Bongini, A.;
2014

Abstract

A structurally correlated series of cell-permeant thiophene fluorophores, characterized by intense green or red fluorescence inside live mouse embryonic fibroblasts, was developed. The fluorophores displayed rapid internalization, excellent retention inside the cells, and high optical stability in the cytosolic environment and did not alter cell viability and reproducibility. Depending on the molecular structure, they experienced distinct fate inside the cells: from bright and lasting staining of the cytoplasm to selective tagging of a small set of globular proteins. © The Royal Society of Chemistry 2014.
2014
Live cell cytoplasm staining and selective labeling of intracellular proteins by non-toxic cell-permeant thiophene fluorophores / DI MARIA, FRANCESCA GIULIA; Palamã , I. E.; Baroncini, Massimo; Barbieri, A.; Bongini, Alessandro; Bizzarri, R.; Gigli, G.; Barbarella, G.. - In: ORGANIC & BIOMOLECULAR CHEMISTRY. - ISSN 1477-0520. - STAMPA. - 12:10(2014), pp. 1603-1610. [10.1039/c3ob41982g]
DI MARIA, FRANCESCA GIULIA; Palamã , I. E.; Baroncini, Massimo; Barbieri, A.; Bongini, Alessandro; Bizzarri, R.; Gigli, G.; Barbarella, G.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/610139
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