Comparative characterization of human and equine Wharton’s jelly derived mesenchymal stem cells

Mesenchymal stem cells (MSCs) have the capability to di erentiate into wide range of specialized cells of mesodermal origin such as osteocytes, chondrocytes, adi- pocytes, cardiomyocytes, muscle bers. Due to these properties, MSCs are considered as a new emerging treatment option and therapeutic agent in regenerative medicine. Promising results have been obtained after application of MSCs for treating tendon and joint disease in the equine model, making it favorable for therapeutic applica- tion. While the horse is considered a highly suitable model for orthopedic diseases, knowledge is lacking regarding the level of analogy of equine MSCs and their human counterparts. Therefore, the aim of this study was to assess the properties of human and equine Wharton’s jelly derived MSCs in a direct comparison. Obtained MSCs, were characterized for their staminal markers, proliferation and adhesion potential, ultrastructural morphology and their ability in di erentiate towards osteogenic, chon- drogenic and adipogenic lineages. Results showed a similar pattern in the expression of staminal markers, while a light di erence was observed in the proliferation and adhesion potential. Ultramorphological analysis showed nuclear and citoplasmatic features comparable in human and equine MSCs. Finally, both MSCs were able to dif- ferentiate towards osteogenic, chondrogenic and adipogenic lineages. In conclusion, although revealing some potentially relevant di erences, the study demonstrates a high level of analogy between human and equine MSCs, providing a basis for trans- lational research in the equine model.