Environmental temperature variations affect many properties and functions of biomolecules and structural components at the cellular level, and influence the animal physiology and behavior at the organism level. It is well accepted that the biochemical changes induced by temperature stress are attributed to gene expression modulations. The aim of this work was to understand the molecular mechanism and the behavioural responses correlated to temperature stress in zebrafish (Danio rerio), which is a widely used animal model for environmental genomics researches. Adult specimens of wild type zebrafish were kept at three different temperatures: 18°C, 34°C and 26°C (used as a control) for 21 days and then subjected to behavioural tests using a YMaze task to evaluate the response to novelty and the spatial memory. Proteomic analysis were carried out on brains to evaluate the thermal effect at the central nervous system level. Briefly, for each temperature 9 brains were lysed and after reduction and derivatization, the proteins were digested with trypsin. LC-ESI MS/MS analysis was performed on a Dionex UltiMate 3000 HPLC. The eluate was electrosprayed into an LTQ Orbitrap Velos. Four technical replicate analyses of each sample were performed. Mass spectra were analyzed using MaxQuant and Peak Studio software. Finally, the bioinformatic analysis was carried out by DAVID and PANTHER softwares to evaluate enriched categories filtered for biological processes, molecular function (MF), cellular component (CC) and pathways involved. Preliminary results suggest that thermal stress at the cellular level influences the CC organization, biogenesis, structural morphogenesis and MF and at the organism level affects the interest for the new environment and the spatial memory.

EFFECT OF THERMAL STRESS ON DANIO RERIO BEHAVIOUR: A PROTEOMIC STUDY TO UNDERSTAND THE MOLECULAR MECHANISM / M. Toni; G. Miccoli; C. Cioni; E. Angiulli; E. Alleva; F. Frabetti; F. Pizzetti; F. Santagata; F. Grassi Scalvini; G. Tedeschi. - In: EUROPEAN JOURNAL OF HISTOCHEMISTRY. - ISSN 2038-8306. - ELETTRONICO. - 61/supplement 1(2017), pp. 30-30.

EFFECT OF THERMAL STRESS ON DANIO RERIO BEHAVIOUR: A PROTEOMIC STUDY TO UNDERSTAND THE MOLECULAR MECHANISM

FRABETTI, FLAVIA;PIZZETTI, FABRIZIO;
2017

Abstract

Environmental temperature variations affect many properties and functions of biomolecules and structural components at the cellular level, and influence the animal physiology and behavior at the organism level. It is well accepted that the biochemical changes induced by temperature stress are attributed to gene expression modulations. The aim of this work was to understand the molecular mechanism and the behavioural responses correlated to temperature stress in zebrafish (Danio rerio), which is a widely used animal model for environmental genomics researches. Adult specimens of wild type zebrafish were kept at three different temperatures: 18°C, 34°C and 26°C (used as a control) for 21 days and then subjected to behavioural tests using a YMaze task to evaluate the response to novelty and the spatial memory. Proteomic analysis were carried out on brains to evaluate the thermal effect at the central nervous system level. Briefly, for each temperature 9 brains were lysed and after reduction and derivatization, the proteins were digested with trypsin. LC-ESI MS/MS analysis was performed on a Dionex UltiMate 3000 HPLC. The eluate was electrosprayed into an LTQ Orbitrap Velos. Four technical replicate analyses of each sample were performed. Mass spectra were analyzed using MaxQuant and Peak Studio software. Finally, the bioinformatic analysis was carried out by DAVID and PANTHER softwares to evaluate enriched categories filtered for biological processes, molecular function (MF), cellular component (CC) and pathways involved. Preliminary results suggest that thermal stress at the cellular level influences the CC organization, biogenesis, structural morphogenesis and MF and at the organism level affects the interest for the new environment and the spatial memory.
M. Toni; G. Miccoli; C. Cioni; E. Angiulli; E. Alleva; F. Frabetti; F. Pizzetti; F. Santagata; F. Grassi Scalvini; G. Tedeschi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/607323
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